Article
Trypanosoma cruzi sensitizes mice to fulminant seb-induced shock: overrelease of inflammatory cytokines and independence of Chagas Disease or TCR Vβ-USAGE
Registro en:
PAIVA, Cláudia N. et al. Trypanosoma cruzi sensitizes mice to fulminant seb-induced shock: overrelease of inflammatory cytokines and independence of Chagas Disease or TCR Vβ-USAGE. Shock, v. 19, n. 2, p. 163-168, 2003.
1073-2322
10.1097/01.SHK.0000054747.80210.e9
1540-0514
Autor
Paiva, Cláudia N.
Pyrrho, Alexandre S.
Lannes-Vieira, Joseli
Vacchio, Melanie
Soares, Milena B. P.
Gattass, Cerli R.
Resumen
Trypanosoma cruzi–infected mice display increased susceptibility to shock induced by injection of lipopolysaccharide (LPS), anti-CD3, or resulting from interleukin (IL)-10-defective response to the parasite itself, but the basis of
such susceptibility remains unknown. Herein, we tested the susceptibility of mice inoculated with virulent and avirulent T.
cruzi to staphylococcal enterotoxins (SE), potent inducers of inflammatory cytokine secretion. Mice infected with T. cruzi
CL-strain or inoculated with the avirulent clone CL-14, a clone that does not induce disease or polyclonal lymphocyte
activation, succumb suddenly to low doses of staphylococcal enterotoxin B (SEB), but not to staphylococcal enterotoxin
A (SEA). High plasma levels of TNF, IFN- , and liver transaminases alanine aminotransferase (ALT) and aspartate
aminotransferase (AST) were found in these mice, indicating lethal toxic shock. Sensitization to shock required inoculation
of live avirulent trypomastigotes and a time interval before challenge with SEB. We found no prior skewing of T cell
receptor (TCR) V-repertoire in CL-14-inoculated mice that could be responsible for sensitization. Splenocytes from
CL-14-inoculated mice proliferated more under anti-V8 than anti-TCR stimulation when compared with normal mice, but
were suppressed to SEB stimulation. Both SEB and anti-V8 antibodies stimulated splenocytes from T. cruzi-inoculated
mice to secrete higher levels of inflammatory cytokines than normal controls. Taken together, our results show that T. cruzi
inoculation can sensitize mice to lethal SEB-induced shock even in the absence of tissue damage, polyclonal lymphocyte
activation, or previously increased levels of inflammatory cytokines, and they suggest that altered reactivity of V8
lymphocytes may be involved in the phenomenon. 2025-01-01