Article
Accuracy of real-time polymerase chain reaction to detectSchistosoma mansoni -infected individuals from an endemic area with low parasite loads
Registro en:
MAGALHÃES, Fernanda do Carmo et al. Accuracy of real-time polymerase chain reaction to detect Schistosoma mansoni – infected individuals from an endemic area with low parasite loads. Parasitology, v. 147, n. 10, 2020.
0031-1820
10.1017/S003118202000089X
Autor
Magalhães, Fernanda do Carmo
Resende, Samira Diniz
Senra, Carolina
Graeff-Teixeira, Carlos
Enk, Martin Johannes
Coelho, Paulo Marcos Zech
Oliveira, Edward Jose de
Negrão-Corrêa, Deborah Aparecida
Geiger, Stefan Michael
Carneiro, Mariângela
Resumen
Due to the efforts to control schistosomiasis transmission in tropical countries, a large proportion of individuals from endemic areas present low parasite loads, which hinders diagnosis of intestinal schistosomiasis by the Kato-Katz (KK) method. Therefore, the development of more sensitive diagnostic methods is essential for efficient control measures. The aim was to evaluate the accuracy of a real-time polymerase chain reaction (RT-PCR) to detectSchistosoma mansoniDNA in fecal samples of individuals with low parasite loads. A cross-sectional population-based study was conducted in a rural community (n= 257) in Brazil. POC-CCA(R)was performed in urine and feces were used for RT-PCR. In addition, fecal exams were completed by 18 KK slides, saline gradient and Helmintex techniques. The combined results of the three parasitological tests detected schistosome eggs in 118 participants (45.9%) and composed the consolidated reference standard (CRS). By RT-PCR, 117 out of 215 tested samples were positive, showing 91.4% sensitivity, 80.2% specificity and good concordance with the CRS (kappa = 0.71). RT-PCR identified 86.9% of the individuals eliminating less than 12 eggs/g of feces, demonstrating much better performance than POC-CCA(R)(50.8%). Our results showed that RT-PCR is a valuable alternative for the diagnosis of intestinal schistosomiasis in individuals with very low parasite loads. 2099-12-31