Article
Characterization of virulence of Leptospira isolates in a hamster model.
Registro en:
SILVA, E. F. et al. Characterization of virulence of Leptospira isolates in a hamster model. Vaccine, v. 26, n. 31, p. 3892-3896, 2008.
0264-410X
Autor
Silva, Éverton Fagonde
Santos, Cleiton Silva
Athanazio, Daniel Abensur
Seyffert, Núbia
Seixas, Fabiana K
Cerqueira, Gustavo Maia de
Fagundes, Michel Q
Brod, Claudiomar Soares
Reis, Mitermayer Galvão dos
Dellagostin, Odir Antonio
Ko, Albert Icksang
Resumen
Effort has been made to identify protective antigens in order to develop a recombinant vaccine against leptospirosis. Several attempts failed to conclusively demonstrate efficacy of vaccine candidates due to the lack of an appropriate model of lethal leptospirosis. The purposes of our study were: (i) to test the virulence of leptospiral isolates from Brazil, which are representative of important serogroups that cause disease in humans and animals; and (ii) to standardize the lethal dose 50% (LD(50)) for each of the virulent strains using a hamster (Mesocricetus auratus) model. Five of seven Brazilian isolates induced lethality in a hamster model, with inocula lower than 200 leptospires. Histopathological examination of infected animals showed typical lesions found in both natural and experimental leptospirosis. Results described here demonstrated the potential use of Brazilian isolates as highly virulent strains in challenge experiments using hamster as an appropriate animal model for leptospirosis. Furthermore these strains may be useful in heterologous challenge studies which aim to evaluate cross-protective responses induced by sub-unit vaccine candidates.