Article
Molecular and biological diversity of HIV-1 in Brazil.
Registro en:
COUTO-FERNANDEZ, J.C. et al. Molecular and biological diversity of HIV-1 in Brazil. Memórias do Instituto Oswaldo Cruz, v. 87, n. 2, p. 249-55, apr.-jun. 1992.
0074-0276
Autor
Fernandez, José Carlos Couto
Morgado, Mariza Gonçalves
Santos, Jairo Ivo dos
Castro Filho, Bernardo Galvão
Resumen
To determine the genomic polymorphism and biological properties present in HIV-1 Brazilian isolates, we analyzed five viral isolates obtained from patients residing in Rio de Janeiro (P1 and P5), São Paulo (P3) and Bahia (P2 and P4) states. For each viral isolate in vitro characteristics such as replication rate, syncytium-inducing capacity and cell death were observed in lymphoblastoid (H9, CEM and peripheral blood mononuclear cells) as well as monocytoid (U937) cells. In addition, the evaluation of the restriction fragment length polymorphism of these isolates was also performed using a panel of endonucleases such as Hind III, Bgl II, Sac I, Pst I, Kpn I and Eco RI. One of the isolates (P1), showed the highest phenotypic and genotypic divergence, when compared to others. The results found suggest a HIV heterogeneity in Brazil similar to that already described in other regions of the world.