Analysis of Schistosoma mansoni population structure using total fecal egg sampling

dc.creatorBlank, Walter A.
dc.creatorReis, Eliana Almeida Gomes
dc.creatorThiong'o, Fred W.
dc.creatorBraghiroli, João F.
dc.creatorSantos, Jarbas M.
dc.creatorMelo, Paulo Roberto Santana de
dc.creatorGuimarães, Isabel C. S.
dc.creatorSilva, Luciano Kalabric
dc.creatorCarmo, Theomira Mauadie de Azevedo
dc.creatorReis, Mitermayer Galvão dos
dc.creatorBlanton, Ronald Edward
dc.date2014-04-14T18:58:05Z
dc.date2014-04-14T18:58:05Z
dc.date2009
dc.date.accessioned2023-09-26T22:45:12Z
dc.date.available2023-09-26T22:45:12Z
dc.identifierBLANK, W. A. et al. Analysis of Schistosoma mansoni population structure using total fecal egg sampling. Journal of Parasitolofy, v. 95, n. 4, p. 881-889, 2009.
dc.identifier1937-2345
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7521
dc.identifier10.1645/GE-1895.1
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8882765
dc.descriptionMany parasite populations are difficult to sample because they are not uniformly distributed between several host species and are often not easily collected from the living host, thereby limiting sample size and possibly distorting the representation of the population. For the parasite Schistosoma mansoni, we investigated the use of eggs, in aggregate, from the stools of infected individuals as a simple and representative sample. Previously, we demonstrated that microsatellite allele frequencies can be accurately estimated from pooled DNA of cloned S. mansoni adults. Here, we show that genotyping of parasite populations from reproductively isolated laboratory strains can be used to identify these specific populations based on characteristic patterns of allele frequencies, as observed by polyacrylamide gel electrophoresis and automated sequencer analysis of fluorescently labeled PCR products. Microsatellites used to genotype aggregates of eggs collected from stools of infected individuals produced results consistent with the geographic distribution of the samples. Preferential amplification of smaller alleles, and stutter PCR products, had negligible effect on measurement of genetic differentiation. Direct analysis of total stool eggs can be an important approach to questions of population genetics for this parasite by increasing the sample size to thousands per infected individual and by reducing bias.
dc.formatapplication/pdf
dc.languageeng
dc.publisherNational Institute of Health
dc.rightsopen access
dc.subjectSchistosoma Mansoni
dc.subjectFecal egg sampling
dc.subjectDNA de Helmintos/química
dc.subjectFezes/parasitologia
dc.subjectRepetições de Microssatélites/genética
dc.subjectSchistosoma mansoni/isolamento & purificação
dc.subjectEsquistossomose mansoni/parasitologia
dc.subjectEletroforese em Gel de Poliacrilamida
dc.subjectAnimais
dc.subjectBrasil
dc.subjectFeminino
dc.subjectFrequência do Gene
dc.subjectGenótipo
dc.subjectHumanos
dc.subjectQuênia
dc.subjectMasculino
dc.subjectÓvulo
dc.subjectReação em Cadeia da Polimerase
dc.subjectSchistosoma mansoni/classificação
dc.subjectSchistosoma mansoni/genética
dc.subjectAnálise de Sequência
dc.titleAnalysis of Schistosoma mansoni population structure using total fecal egg sampling
dc.typeArticle


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