Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis

dc.creatorMarcos, Caroline Maria
dc.creatorOliveira, Haroldo Cesar de
dc.creatorAssato, Patrícia Akemi
dc.creatorCastelli, Rafael Fernando
dc.creatorFusco-Almeida, Ana Marisa
dc.creatorMendes-Giannini, Maria José Soares
dc.date2021-11-25T16:47:59Z
dc.date2021-11-25T16:47:59Z
dc.date2021
dc.date.accessioned2023-09-26T22:44:37Z
dc.date.available2023-09-26T22:44:37Z
dc.identifierMARCOS, Caroline Maria et al. Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis. J. Fungi, V.7, n. 852, p. 1–22, 2021.
dc.identifier2309-608X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/50063
dc.identifier10.3390/jof7100852
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8882641
dc.descriptionP. brasiliensis is a thermally dimorphic fungus belonging to Paracoccidioides complex, causative of a systemic, endemic mycosis limited to Latin American countries. Signal transduction pathways related to important aspects as surviving, proliferation according to the biological niches are linked to the fungal pathogenicity in many species, but its elucidation in P. brasiliensis remains poorly explored. As Drk1, a hybrid histidine kinase, plays regulators functions in other dimorphic fungi species, mainly in dimorphism and virulence, here we investigated its importance in P. brasilensis. We, therefore generated the respective recombinant protein, anti-PbDrk1 polyclonal antibody and a silenced strain. The Drk1 protein shows a random distribution including cell wall location that change its pattern during osmotic stress condition; moreover the P. brasiliensis treatment with anti-PbDrk1 antibody, which does not modify the fungus’s viability, resulted in decreased virulence in G. mellonella model and reduced interaction with pneumocytes. Down-regulating PbDRK1 yielded phenotypic alterations such as yeast cells with more elongated morphology, virulence attenuation in G. mellonella infection model, lower amount of chitin content, increased resistance to osmotic and cell wall stresses, and also caspofungin, and finally increased sensitivity to itraconazole. These observations highlight the importance of PbDrk1 to P. brasiliensis virulence, stress adaptation, morphology, and cell wall organization, and therefore it an interesting target that could help develop new antifungals
dc.formatapplication/pdf
dc.languagepor
dc.publisherMDPI
dc.rightsopen access
dc.subjectDrk1
dc.subjectHistidine Kinase
dc.subjectParacoccidioides brasiliensis
dc.subjectBroadly Neutralizing Antibodies
dc.subjectRNA Silencing
dc.subjectCell Wall
dc.subjectVirulence
dc.subjectHistidina Quinasa
dc.subjectParacoccidioides
dc.subjectAnticuerpos ampliamente neutralizantes
dc.subjectInterferencia de ARN
dc.subjectPared Celular
dc.subjectVirulencia
dc.subjectHistidine kinase
dc.subjectParacoccidioides
dc.subjectInterférence par ARN
dc.subjectParoi cellulaire
dc.subjectHistidina Quinase
dc.subjectParacoccidioides
dc.subjectAnticorpos Amplamente Neutralizantes
dc.subjectInterferência de RNA
dc.subjectParede Celular
dc.subjectVirulência
dc.titleDrk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis
dc.typeArticle


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