Article
Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro
Registro en:
PAIVA, Lígia Almeida; et al. Hepatic myofibroblasts derived from Schistosoma mansoni-infected mice are a source of IL-5 and eotaxin: controls of eosinophil populations in vitro. Parasites & Vectors, v.8:577, 10p, 2015.
1756-3305
10.1186/s13071-015-1197-3
Autor
Paiva, Ligia Almeida
Brand, Camila
Melo, Christianne Bandeira
Bozza, Patrícia Torres
El-Cheikin, Marcia Cury
Silva, Patrícia Martins
Borojevic, Radovan
Perez, Sandra Aurora Chavez
Resumen
Background: Hepatic myofibroblasts are relevant for pathogenesis of S. mansoni infection. In normal liver, these
perisinusoidal cells are quiescent, express the lipocyte phenotype, and are located in the Disse’s space, being the
major site of vitamin A storage. When activated, they convert to myofibroblasts and contribute to granulomatous
and diffuse liver fibrosis. In the present work, we observed that myofibroblasts obtained from granulomatous periovular
inflammatory reactions in schistosome-infected mice (GR-MF) produce in vitro immunomodulatory cytokines for
eosinophil activation: IL-5 and eotaxin.
Methods and results: The secretory activity of GR-MF was detected after TGF-β and IL-13 stimulation using 2D and 3D
cell culture systems. In a mixed co-culture system using GR-MF with hematopoietic bone marrow cells from infected
mice, we observed eosinophil survival that was dependent upon IL-5 and eotaxin, since antibodies against this cytokines
decreased eosinophil population, as measured by eosinophil peroxidase activity.
Conclusion: These results indicate that GR-MF may contribute to maintenance of local eosinophilia in schistosomal
hepatic granulomas, and can function as immunoregulatory cells, besides their role in production of fibrosis.