Genetic and biological characterisation of Zika virus isolates from different brazilian regions

dc.creatorStrottmann, Daisy Maria
dc.creatorZanluca, Camila
dc.creatorMosimann, Ana Luiza Pamplona
dc.creatorKoishi, Andrea Cristine
dc.creatorAuwerter, Nathalia Cavalheiro
dc.creatorFaoro, Helisson
dc.creatorCataneo, Allan Henrique Depieri
dc.creatorKuczera, Diogo
dc.creatorWowk, Pryscilla Fanini
dc.creatorBordignon, Juliano
dc.creatorSantos, Claudia Nunes Duarte dos
dc.date2019-09-13T19:13:42Z
dc.date2019-09-13T19:13:42Z
dc.date2019
dc.date.accessioned2023-09-26T22:24:39Z
dc.date.available2023-09-26T22:24:39Z
dc.identifierSTROTTMANN, Daisy Maria et al. Genetic and biological characterisation of Zika virus isolates from different Brazilian regions. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-11, 2019.
dc.identifier1678-8060
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/35589
dc.identifier10.1590/0074-02760190150
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8878898
dc.descriptionZika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 brazilian epidemics. As methods for this study, the ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. As findings, are eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the ellestablished ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. As main conclusions had that Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells.
dc.formatapplication/pdf
dc.languagepor
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subjectFlavivirus
dc.subjectGenoma Viral
dc.subjectZika Virus
dc.subjectGenetic Markers
dc.subjectWhole Genome Sequencing
dc.subjectVirus Activation
dc.subjectVirus Zika
dc.subjectSecuenciación Completa del Genoma
dc.subjectActivación Viral
dc.subjectZika Virus
dc.subjectMarcadores Genéticos
dc.subjectSequenciamento Completo do Genoma
dc.subjectAtivação Viral
dc.titleGenetic and biological characterisation of Zika virus isolates from different brazilian regions
dc.typeArticle


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