Article
Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests
Registro en:
TEIXEIRA, Ana Izabel Passarella et al. Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-9, 2019.
0074-0276
10.1590/0074-02760180452
Autor
Teixeira, Ana Izabel Passarella
Silva, Debora Marcolino
Vital, Tamires
Nitz, Nadjar
Carvalho, Bruna Caroline de
Hecht, Mariana
Oliveira, Diana Souza de
Oliveira, Edward Jose de
Rabello, Ana Lucia Teles
Romero, Gustavo Adolfo Sierra
Resumen
BACKGROUND: Studies aimed at validating canine visceral leishmaniasis diagnostic tests present heterogeneous results regarding test accuracy, partly due to divergences in reference standards used and different infection evolution periods in animals.
OBJECTIVE: This study aimed to evaluate the accuracy of the rapid test-dual path platform (TR-DPP) (Biomanguinhos®), EIE-Leishmaniose-Visceral-Canina-Biomanguinhos (EIE-LVC) (Biomanguinhos®), enzyme-linked immunosorbent assay (ELISA) rK39 (in-house), and the direct agglutination test (DAT-Canis) against a reference standard comprising parasitological and molecular techniques.
METHODS: A phase II/III validation study was carried out in sample sera from 123 predominantly asymptomatic dogs living in an area endemic for visceral leishmaniasis.
FINDINGS: Sixty-nine (56.1%) animals were considered infected according to the reference standard. For each test, the sensitivity and specificity, respectively, were as follows: TR-DPP, 21.74% [confidence interval (CI)95% 13.64% to 32.82%] and 92.59% (CI95% 82.45% to 97.08%); EIE-LVC, 11.59% (CI95% 5.9% to 21.25%) and 90.74% (CI95% 80.09% to 95.98%); ELISA rK39, 37.68% (CI95% 27.18% to 49.48%) and 83.33% (CI95% 71.26% to 90.98%); and DAT-Canis, 18.84% (CI95% 11.35% to 29.61%) and 96.30% (CI95% 87.46% to 98.98%).
CONCLUSION: We concluded that improving the sensitivity of serum testing for diagnosing asymptomatic dogs must constitute a priority in the process of developing new diagnostic tests to be used in the visceral leishmaniasis control program in Brazil.