This research was supported by FEDER funds through the Operational Programme COMPETE and national funds through Fundação para a Ciência e Tecnologia, in the scope of project PEst-C/BIA/UI4050/ 2011, and by FAPERJ/Rio de Janeiro, Brazil (grant proc. E-26/110.619/ 2012) R. M. Z-O. is supported in part by CNPq 350338/2000-0 and FAPERJ E-26/103.157/2011. M. M. E. O. was supported by a grant from CAPES 2445/11-5.In this study, we characterize the first autochthone case of human sporotrichosis reported in Lisbon, Portugal. Phenotypic and genotypic characterization revealed that the infection was caused by Sporothrix globosa. We conclude that sporotrichosis may be underdiagnosed particularly in Southern Europe and suggest Portugal as an emerging area for this fungal infection.