Article
Differential Gel Electrophoresis (DIGE) Evaluation of Naphthoimidazoles Mode of Action: A Study in Trypanosoma cruzi Bloodstream Trypomastigotes
Registro en:
BRUNORO, Giselle Villa Flor; et al. Differential Gel Electrophoresis (DIGE) Evaluation of Naphthoimidazoles Mode of Action: A Study in Trypanosoma cruzi Bloodstream Trypomastigotes. PLoS Negl Trop Dis., v.10, n.8, e0004951., 18p, 2016.
1935-2727
10.1371/journal.pntd.0004951
1935-2735)
Autor
Brunoro, Giselle Villa Flor
Faça, Vitor Marcel
Caminha, Marcelle Almeida
Ferreira, André Teixeira da Silva
Trugilho, Monique
Moura, Kelly Cristina Gallan de
Perales, Jonas
Valente, Richard Hemmi
Menna-Barreto, Rubem Figueiredo Sadok
Resumen
The obligate intracellular protozoan Trypanosoma cruzi is the causative agent of Chagas disease, a neglected illness affecting millions of people in Latin America that recently entered non-endemic countries through immigration, as a consequence of globalization. The chemotherapy for this disease is based mainly on benznidazole and nifurtimox, which are very efficient nitroderivatives against the acute stage but present limited efficacy during the chronic phase. Our group has been studying the trypanocidal effects of naturally occurring quinones and their derivatives, and naphthoimidazoles derived from β-lapachone N1, N2 and N3 were the most active. To assess the molecular mechanisms of action of these compounds, we applied proteomic techniques to analyze treated bloodstream trypomastigotes, which are the clinically relevant stage of the parasite.