Article
Toxicity of terpenes on fibroblast cells compared to their hemolytic potential and increase in erythrocyte membrane fluidity
Registro en:
MENDANHA, Sebastião A. et al. Toxicity of terpenes on fibroblast cells compared to their hemolytic potentialand increase in erythrocyte membrane fluidity. Toxicology in Vitro, v. 27, p. 323-329, 2013.
0887-2333
10.1016/j.tiv.2012.08.022
1879-3177
Autor
Mendanha, Sebastião A.
Moura, Soraia S.
Anjos, Jorge L. V.
Valadares, Marize C.
Alonso, Antonio A.
Resumen
Soraia S. Moura. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento. erpenes are considered potent skin permeation enhancers with low toxicity. Electron paramagnetic res-onance (EPR) spectroscopy of the spin label 5-doxyl stearic acid (5-DSA) was used to monitor the effect ofsesquiterpene nerolidol and various monoterpenes on membrane fluidity in erythrocyte and fibroblastcells. In addition, the hemolytic levels and cytotoxic effects on cultured fibroblast cells were also mea-sured to investigate possible relationships between the cellular irritation potentials of terpenes andthe ability to modify membrane fluidity. All terpenes increased cell membrane fluidity with no significantdifferences between the monoterpenes, but the effect of sesquiterpene was significantly greater than thatof the monoterpenes. The IC50values for the terpenes in the cytotoxicity assay indicated that 1,8-cineoleshowed lower cytotoxicity anda-terpineol and nerolidol showed higher cytotoxicity. The correlationbetween the hemolytic effect and the IC50values for fibroblast viability was low (R= 0.61); however,in both tests, nerolidol was among the most aggressive of terpenes and 1,8-cineole was among the leastaggressive. Obtaining information concerning the toxicity and potency of terpenes could aid in the designof topical formulations optimized to facilitate drug absorption for the treatment of many skin diseases. 2020-10-01