Article
Production of monoclonal antibodies against canine leukocytes.
Registro en:
AGUIAR, P. H. P. et al. Production of monoclonal antibodies against canine leukocytes. Hybrid Hybridomics, v. 23, n. 2, p. 127-32, apr. 2004.
1536-8599
Autor
Aguiar, Paulo Henrique Palis
Santos, Roberto Robson Borges dos
Lima, Carla Andrade
Gomes, Hilton Rios de Sousa
Larangeira, Daniela Farias
Santos, Patrícia Oliveira Meira
Melo, Stella Maria Barrouin
dosSantos, Washington Luis Conrado
Pontes-de-Carvalho, Lain Carlos
Resumen
A panel of anti-canine leukocyte monoclonal antibodies (MAbs) was produced by immunizing BALB/c mice
with canine peripheral blood mononuclear cells (PBMC), either resting or stimulated with concanavalin A
(ConA). Three out of 28 clones—IH1, AB6, and HG6–screened by ELISA and producing antibody with the
highest specificity for canine cell immunostaining, were subjected to three subsequent subcloning steps by limiting
dilution, and selected for further characterization. These MAbs belonged to IgG1 (HG6 and IH1) and
IgG2a (AB6) isotypes. The distribution of cell populations expressing the antigen recognized by the antibodies
was identified by indirect immunoflorescence on canine PBMC and on tissue sections of lymph node, spleen,
liver and skin. The possible crossreactivity with human PBMC was also examined in immunocytochemistry.
One of the antibodies specifically recognized macrophages. The MAbs presented here can be foreseen as possible
valuable diagnostic and research tools to study immune functions in dogs.