Plasma superoxide dismutase-1 as a surrogate marker of vivax malaria severity

dc.creatorAndrade, Bruno de Bezerril
dc.creatorReis Filho, Antonio José Souza
dc.creatorSouza Neto, Sebastião Martins
dc.creatorRaffaele Netto, Imbroinise
dc.creatorCamargo, Luís Marcelo Aranha
dc.creatorBarral, Aldina Maria Prado
dc.creatorBarral Netto, Manoel
dc.date2014-03-06T17:29:04Z
dc.date2014-03-06T17:29:04Z
dc.date2010
dc.date.accessioned2023-09-26T22:11:13Z
dc.date.available2023-09-26T22:11:13Z
dc.identifierANDRADE, B. B. et al. Plasma superoxide dismutase-1 as a surrogate marker of vivax malaria severity. Plos Neglected Tropical Diseases, v. 4, p. e650, 2010.
dc.identifier1935-2735
dc.identifier10.1371/journal.pntd.0000650
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7378
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8875952
dc.descriptionSevere outcomes have been described for both Plasmodium falciparum and P. vivax infections. The identification of sensitive and reliable markers of disease severity is fundamental to improving patient care. An intense pro-inflammatory response with oxidative stress and production of reactive oxygen species is present in malaria. Inflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) and antioxidant agents such as superoxide dismutase-1 (SOD-1) are likely candidate biomarkers for disease severity. Here we tested whether plasma levels of SOD-1 could serve as a biomarker of severe vivax malaria. METHODOLOGY/PRINCIPAL FINDINGS: Plasma samples were obtained from residents of the Brazilian Amazon with a high risk for P. vivax transmission. Malaria diagnosis was made by both microscopy and nested PCR. A total of 219 individuals were enrolled: non-infected volunteers (n = 90) and individuals with vivax malaria: asymptomatic (n = 60), mild (n = 50) and severe infection (n = 19). SOD-1 was directly associated with parasitaemia, plasma creatinine and alanine amino-transaminase levels, while TNF-alpha correlated only with the later enzyme. The predictive power of SOD-1 and TNF-alpha levels was compared. SOD-1 protein levels were more effective at predicting vivax malaria severity than TNF-alpha. For discrimination of mild infection, elevated SOD-1 levels showed greater sensitivity than TNF-alpha (76% vs. 30% respectively; p<0.0001), with higher specificity (100% vs. 97%; p<0.0001). In predicting severe vivax malaria, SOD-1 levels exhibited higher sensitivity than TNF-alpha (80% vs. 56%, respectively; p<0.0001; likelihood ratio: 7.45 vs. 3.14; p<0.0001). Neither SOD-1 nor TNF-alpha could discriminate P. vivax infections from those caused by P. falciparum. CONCLUSION: SOD-1 is a powerful predictor of disease severity in individuals with different clinical presentations of vivax malaria.
dc.formatapplication/pdf
dc.languageeng
dc.publisherNew York University School of Medicine
dc.rightsopen access
dc.subjectMalária Vivax/diagnóstico
dc.subjectMalária Vivax/patologia
dc.subjectSuperóxido Dismutase/sangue
dc.subjectAdolescente
dc.subjectAdulto
dc.subjectAnimais
dc.subjectMarcadores Biológicos
dc.subjectBrasil
dc.subjectFeminino
dc.subjectHumanos
dc.subjectMasculino
dc.subjectMicroscopia
dc.subjectMeia-Idade
dc.subjectParasitemia
dc.subjectPlasma/química
dc.subjectPlasmodium vivax/imunologia
dc.subjectPlasmodium vivax/isolamento & purificação
dc.subjectReação em Cadeia da Polimerase
dc.subjectValor Preditivo dos Testes
dc.subjectPrognóstico
dc.subjectAdulto Jovem
dc.titlePlasma superoxide dismutase-1 as a surrogate marker of vivax malaria severity
dc.typeArticle


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