Article
Frozen blood clots can be used for the diagnosis of distinct Plasmodium species in man and non‑human primates from the Brazilian Atlantic Forest
Registro en:
ABREU, Filipe Vieira Santos de et al. Frozen blood clots can be used for the diagnosis of distinct Plasmodium species in man and non‑human primates from the Brazilian Atlantic Forest. Malaria Journal, p. 1-5, 2018.
1475-2875
10.1186/s12936-018-2485-0
1475-2875
Autor
Abreu, Filipe Vieira Santos de
Gomes, Larissa Rodrigues
Mello, Aline Rosa Lavigne
Bianco‑Júnior, Cesare
Pina‑Costa, Anielle
Santos, Edmilson dos
Teixeira, Danilo Simonini
Brasil, Patrícia
Daniel‑Ribeiro, Cláudio Tadeu
Lourenço‑de‑Oliveira, Ricardo
Ferreira‑da‑Cruz, Maria de Fátima
Resumen
2019 - Correction to: Frozen blood clots can be used for the diagnosis of distinct Plasmodium species in man and non-human primates from the Brazilian Atlantic Forest - Following publication of the original article [1], it was flagged that one of the authors (Anielle de Pina Costa) is missing an affiliation in the article. Please note the author Anielle de Pina Costa also has this affiliation: Centro Universitário Serra dos Órgãos (UNIFESO), Teresópolis/RJ, 25964-004, Brazil. The author apologizes for this error.
DOI 10.1186/s12936-019-2804-0 Background: Zoonotic infections with epidemic potential, as non-human primate malaria and yellow fever (YF), can overlap geographically. Optimizing a small blood sample for diagnosis and surveillance is of great importance. Blood are routinely collected for YF diagnosis and blood clots usually discarded after serum obtention. Aiming to take sample advantage, the sensitivity of a PCR using extracted DNA from long-term frozen clots from human and non-human primates for detection of Plasmodium spp. in low parasitaemia conditions was assayed.
Results: Malaria diagnosis with DNA extracted from blood clots generated results in agreement with samples
obtained with whole blood, including mixed Plasmodium vivax/simium and Plasmodium malariae/brasilianum
infections.
Conclusion: Blood clots from human and non-human primates may be an important and low cost source of DNA for malaria surveillance in the Atlantic Forest.