Crystal structure of the MIF4G domain of the Trypanosoma cruzi translation initiation factor EIF4G5

dc.creatorSantos, Lucca Pietro Camillo dos
dc.creatorMatos, Bruno Moisés de
dc.creatorRibeiro, Brenda Cecilia de Maman
dc.creatorZanchin, Nilson Ivo Tonin
dc.creatorGuimarães, Beatriz Gomes
dc.date2020-12-10T16:59:10Z
dc.date2020-12-10T16:59:10Z
dc.date2019
dc.date.accessioned2023-09-26T21:27:47Z
dc.date.available2023-09-26T21:27:47Z
dc.identifierSANTOS, Lucca Pietro Camillo dos. Crystal structure of the MIF4G domain of the Trypanosoma cruzi translation initiation fator EIF4G5. Acta Cryst. n.75, p. 738–743.
dc.identifier2053-230X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44789
dc.identifier10.1107/S2053230X19015061
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8872482
dc.descriptionKinetoplastida, a class of early-diverging eukaryotes that includes pathogenic Trypanosoma and Leishmania species, display key differences in their translation machinery compared with multicellular eukaryotes. One of these differences involves a larger number of genes encoding eIF4E and eIF4G homologs and the interaction pattern between the translation initiation factors. eIF4G is a scaffold protein which interacts with the mRNA cap-binding fator eIF4E, the poly(A)-binding protein, the RNA helicase eIF4A and the eIF3 complex. It contains the so-called middle domain of eIF4G (MIF4G), a multipurpose adaptor involved in different protein–protein and protein–RNA complexes. Here, the crystal structure of the MIF4G domain of T. cruzi EIF4G5 is described at 2.4 A˚ resolution, which is the first three-dimensional structure of a trypanosomatid MIF4G domain to be reported. Structural comparison with IF4G homologs from other eukaryotes and other MIF4G-containing proteins reveals differences that may account for the specific interaction mechanisms of MIF4G despite its highly conserved overall fold.
dc.formatapplication/pdf
dc.languagepor
dc.publisherInternational Union of Crystallography
dc.rightsopen access
dc.subjectPeptide Chain Initiation, Translational
dc.subjectEukaryotic Initiation Factor-4G
dc.subjectIniciación de la Cadena Peptídica Traduccional
dc.subjectFactor 4G Eucariótico de Iniciación
dc.subjectInitiation de la traduction
dc.subjectFacteur-4G d'initiation eucaryote
dc.subjectTrypanosoma cruzi
dc.subjectKinetoplastida
dc.subjectIniciação Traducional da Cadeia Peptídica
dc.subjectFator de Iniciação 4G em Eucariotos
dc.titleCrystal structure of the MIF4G domain of the Trypanosoma cruzi translation initiation factor EIF4G5
dc.typeArticle


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