Article
N-terminal amino acid sequences of the major outer membrane proteins from a Neisseria meningitidis group B strain isolated in Brazil
Registro en:
DE SIMONE, Salvatore Gopvanni et al. N-terminal amino acid sequences of the major outer membrane proteins from a Neisseria meningitidis group B strain isolated in Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 91, n. 1, p. 111-116, Jan./Feb. 1996.
0074-0276
10.1590/S0074-02761996000100020
1678-8060
Autor
De Simone, Salvatore Giovanni
Soares, Solange A. F.
Souza, Andre L. A.
Danelli, Maria G. M.
Resumen
The four dominant outer membrane proteins (46, 38, 33 and 28 kDa) were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in a semi-purified preparation of vesicle membranes of a Neisseria meningitidis (N44/89, B:4:P1.15:P5.5,7) strain isolated in Brazil. The N-terminal amino acid sequence for the 46 kDa and 28 kDa proteins matched that reported by others for class 1 and 5 proteins respectively, whereas the sequence (25 amino acids) for the 38 kDa (class 3) protein was similar to class 1 meningococcal proteins. The sequence for the 33 kDa (class 4) was unique and not homologous to any known protein.