Article
Associations between TGF-β1 Levels and Markers of Hemolysis, Inflammation, and Tissue Remodeling in Pediatric Sickle Cell Patients
Registro en:
SANTIAGO, Rayra P. et al. Associations between TGF-β1 Levels and Markers of Hemolysis, Inflammation, and Tissue Remodeling in Pediatric Sickle Cell Patients. Mediators of Inflammation, 2021.
0962-9351
10.1155/2021/4651891
Autor
Santiago, Rayra P.
Carvalho, Magda O. S.
Figueiredo, Camylla V. B.
Fiuza, Luciana M.
Oliveira, Rodrigo M.
Yahouédéhou, Sètondji Cocou Modeste Alexandre
Nascimento, Valma M. L.
Lyra, Isa M.
Santos, Théo Araujo
Luz, Nívea F.
Aleluia, Milena M.
Guarda, Caroline Conceição
Borges, Valéria de Matos
Goncalves, Marilda de Souza
Resumen
Coordenação
de Aperfeiçoamento de Pessoal de Nível Superior-Brasil
(CAPES), Finance Code 001 (RPS and SCMAY). Our work
was also supported by the Fundação de Amparo à Pesquisa
do Estado da Bahia (FAPESB SUS0034/2013 and 8133/2014)
through a grant to MSG. Transforming growth factor beta (TGF-β) is a cytokine with important involvement in biological processes related to the pathogenesis
of sickle cell disease (SCD), including endothelial and vascular dysfunction, inflammation, and hematopoietic homeostasis. This study
is aimed at investigating associations between levels of TGF-β1 and classical laboratory biomarkers and inflammatory mediators, as
well as the tissue inhibitor of metalloproteases-1 (TIMP-1) and matrix metalloproteinase-9 (MMP-9), in pediatric patients (n = 123)
with SCD in steady state: 84 with sickle cell anemia (HbSS) and 39 with hemoglobin SC disease (HbSC). A healthy control (HC) group
of 59 individuals was also included. Hematological and biochemical analyses were carried out using electronic methods. TGF-β1,
TIMP-1, and MMP-9 plasma quantifications were performed by ELISA. TGF-β1 plasma levels were higher in HbSS individuals
than in HbSC and HC. In individuals with HbSS, TGF-β1 levels were positively correlated with red blood cells, hemoglobin,
hematocrit, platelets, and TIMP-1. In addition, HbSS individuals with TGF-β1 levels above the median (≥72.29 ng/mL) also
presented increased monocyte counts and decreased albumin levels. In patients with HbSC, TGF-β1 levels were positively
correlated with leukocytes, eosinophils, lymphocytes, monocytes, and platelets, as well as levels of TIMP-1, VLDL-C, triglycerides,
heme, and AST. Additionally, HbSC individuals with TGF-β1 levels above the median (≥47.80 ng/mL) presented increased
leukocyte and platelet counts, as well as increased levels of triglycerides, VLDL-C, MMP-9, and TIMP-1, and decreased HDL-C.
Our findings suggest that TGF-β1 may play important roles in vascular remodeling, vasculopathy, angiogenesis, and inflammation
in pediatric patients with SCD.