Czech Science Foundation (project 17-103083 S; https://gacr.cz/en/) - IR, MS, PV, Grant Agency of Charles University (GAUK 1642314/2014 - TL, IR, UNCE 204017/2012 - TL, MS).Leishmaniases are parasitic diseases present worldwide that are transmitted to the vertebrate host by the bite of an infected sand fly during a blood feeding. Phlebotomine sand flies inoculate into the mammalian host Leishmania parasites embedded in promastigote secretory gel (PSG) with saliva, which is composed of a diverse group of molecules with pharmacological and immunomodulatory properties.