Article
Leishmania-Induced Dendritic Cell Migration and Its Potential Contribution to Parasite Dissemination
Registro en:
REBOUÇAS, Amanda et al. Leishmania-Induced Dendritic Cell Migration and Its Potential Contribution to Parasite Dissemination. Microorganisms, 2021.
2076-2607
10.3390/microorganisms9061268
Autor
Rebouças, Amanda
Silva, Thaílla S.
Medina, Lilian S.
Paredes, Bruno D.
Aragão, Luciana S.
Borges, Valéria de Matos
Schriefer, Albert
Veras, Patrícia Sampaio Tavares
Brodskyn, Claudia Ida
Juliana Perrone Bezerra de Menezes
Resumen
Conselho Nacional de Desenvolvimento
Científico e Tecnológico (CNPq), Programa Inova Fiocruz and National Institute of Health (NIH)
grant AI136032. C.I.B., V.M.B. and P.S.T.V. are senior investigators of CNPq. P.S.T.V. holds a grant
from CNPq for productivity in research (307832/2015-5). Leishmania, an intracellular parasite species, causes lesions on the skin and in the mucosa
and internal organs. The dissemination of infected host cells containing Leishmania is crucial to
parasite survival and the establishment of infection. Migratory phenomena and the mechanisms
underlying the dissemination of Leishmania-infected human dendritic cells (hDCs) remain poorly
understood. The present study aimed to investigate differences among factors involved in hDC
migration by comparing infection with visceral leishmaniasis (VL) induced by Leishmania infantum
with diverse clinical forms of tegumentary leishmaniasis (TL) induced by Leishmania braziliensis or
Leishmania amazonensis. Following the infection of hDCs by isolates obtained from patients with
different clinical forms of Leishmania, the formation of adhesion complexes, actin polymerization,
and CCR7 expression were evaluated. We observed increased hDC migration following infection
with isolates of L. infantum (VL), as well as disseminated (DL) and diffuse (DCL) forms of cutaneous
leishmaniasis (CL) caused by L. braziliensis and L. amazonensis, respectively. Increased expression
of proteins involved in adhesion complex formation and actin polymerization, as well as higher
CCR7 expression, were seen in hDCs infected with L. infantum, DL and DCL isolates. Together, our
results suggest that hDCs play an important role in the dissemination of Leishmania parasites in the
vertebrate host.