Article
Serological reactivity against T. cruzi-derived antigens: Evaluation of their suitability for the assessment of response to treatment in chronic Chagas disease
Registro en:
ALONSO-PADILLA, Julio et al. Serological reactivity against T. cruzi-derived antigens: Evaluation of their suitability for the assessment of response to treatment in chronic Chagas disease. Acta Tropica, v. 221, p. 1-10, 2021.
0001-706X
10.1016/j.actatropica.2021.105990
Autor
Alonso-Padilla, Julio
López, Manuel Carlos
Esteva, Mónica
Zrein, Maan
Casellas, Aina
Gómez, Inmaculada
Granjon, Elodie
Méndez, Susana
Benítez, Celia
Ruiz, Andres Mariano
Sanz, Sergi
Gascón, Joaquim
Thomas, M. Carmen
Pinazo, Maria-Jesus
Abril, Marcelo
Noya, Belkisyolé Alarcón de
Jorge, Tania Araujo
Chatelain, Eric
Grijalva, Mario J.
Guhl, Felipe
Hasslocher-Moreno, Alejandro Marcel
Luquetti, Alejandro O.
Noya, Oscar
Ramsey, Janine M.
Ribeiro, Isabela
Longhi, Silvia A.
Schijman, Alejandro G.
Sosa-Estani, Sergio
Torrico, Faustino
Viotti, Rodolfo
Resumen
Chagas disease, caused by the protozoan Trypanosoma cruzi, affects more than 6 million people worldwide. Following a mostly asymptomatic acute phase, the disease progresses to a long-lasting chronic phase throughout which life-threatening disorders to the heart and/or gastrointestinal tract will manifest in about 30% of those chronically infected. During the chronic phase, the parasitemia is low and intermittent, while a high level of anti-T. cruzi antibodies persist for years. These two features hamper post-chemotherapeutic follow-up of patients with the tools available. The lack of biomarkers for timely assessment of therapeutic response discourages a greater use of the two available anti-parasitic drugs, and complicates the evaluation of new drugs in clinical trials. Herein, we investigated in a blinded case-control study the serological reactivity over time of a group of parasite-derived antigens to potentially address follow up of T. cruzi chronically infected subjects after treatment. We tested PFR2, KMP11, HSP70, 3973, F29 and the InfYnity multiplexed antigenic array, by means of serological assays on a multi-national retrospective collection of samples. Some of the antigens exhibited promising results, underscoring the need for further studies to determine their potential role as treatment response biomarkers.