Cramoll 1,4 lectin increases ROS production, calcium levels, and cytokine expression in treated spleen cells of rats

dc.creatorMelo, Cristiane Moutinho Lagos de
dc.creatorPaim, Bruno Alves
dc.creatorZecchin, Karina Gotardelo
dc.creatorMorari, Joseani
dc.creatorChiaratti, Marcos R.
dc.creatorCorreia, Maria Tereza Santos
dc.creatorCoelho, Luana Cassandra B. Barroso
dc.creatorPaiva, Patrícia Maria Guedes
dc.date2018-05-03T13:26:34Z
dc.date2018-05-03T13:26:34Z
dc.date2010
dc.date.accessioned2023-09-26T20:56:17Z
dc.date.available2023-09-26T20:56:17Z
dc.identifierDE MELO, C. M. L. et al. Cramoll 1,4 lectin increases ROS production, calcium levels, and cytokine expression in treated spleen cells of rats. Molecular and Cellular Biochemistry, v. 342, n. 1–2, p. 163–169, set. 2010.
dc.identifier1573-4919
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/26231
dc.identifier10.1007/s11010-010-0480-z
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8866472
dc.descriptionEste trabalho foi financiado pela Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), pelo Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) e pela Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
dc.descriptionThis study reports the in vivo stimulatory effects of Cramoll 1,4 on rat spleen lymphocytes as evidenced by an increase in intracellular reactive oxygen species (ROS) production, Ca(2+) levels, and interleukin (IL)-1beta expression. Cramoll 1,4 extracted from seeds of the Leguminosae Cratylia mollis Mart., is a lectin with antitumor and lymphocyte mitogenic activities. Animals (Nine-week-old male albino Wistar rats, Rattus norvegicus) were treated with intraperitoneal injection of Cramoll 1,4 (235 microg ml(-1) single dose) and, 7 days later, spleen lymphocytes were isolated and analyzed for intracellular ROS, cytosolic Ca(2+), and IL-6, IL-10, and IL-1 mRNAs. Cell viability was investigated by annexin V-FITC and 7-amino-actinomycin D staining. The data showed that in lymphocytes activated by Cramoll 1,4 the increase in cytosolic and mitochondrial ROS was related to higher cytosolic Ca(2+) levels. Apoptosis and necrosis were not detected in statistically significant values and thus the lectin effector activities did not induce lymphocyte death. In vivo Cramoll 1,4 treatment led to a significant increase in IL-1beta but IL-6 and -10 levels did not change. Cramoll 1,4 had modulator activities on spleen lymphocytes and stimulated the Th2 response.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectCratylia mollis
dc.subjectCálcio
dc.subjectInterleucina
dc.subjectLectina
dc.subjectEspécies reativas de oxigênio
dc.subjectCratylia mollis
dc.subjectCalcium
dc.subjectInterleukin
dc.subjectLectin
dc.subjectReactive oxygen species
dc.subjectAnimais
dc.subjectCálcio / metabolismo
dc.subjectCélulas Cultivadas
dc.subjectInjeções intraperitoneais
dc.subjectInterleucina-10 / metabolismo
dc.subjectInterleucina-1 beta / metabolismo
dc.subjectInterleucina-6 / metabolismo
dc.subjectLinfócitos / citologia
dc.subjectLinfócitos / efeitos de drogas
dc.subjectLinfócitos / metabolismo
dc.subjectMasculino
dc.subjectLectinas vegetais / isolamento e purificação
dc.subjectLectinas vegetais / farmacologia
dc.subjectRatos
dc.subjectRatos Wistar
dc.subjectEspécies de Oxigênio Reativas / metabolismo
dc.subjectBaço / citologia
dc.subjectBaço / efeitos de drogas
dc.subjectBaço / metabolismo
dc.titleCramoll 1,4 lectin increases ROS production, calcium levels, and cytokine expression in treated spleen cells of rats
dc.typeArticle


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