Mitochondrial permeability transition pore in sea urchin female gametes

dc.creatorTorrezan-Nitao, Elis
dc.creatorFigueiredo, Regina Celia Bressan Queiroz
dc.creatorMarques-Santos, Luis Fernando
dc.date2019-11-11T14:31:42Z
dc.date2019-11-11T14:31:42Z
dc.date2018
dc.date.accessioned2023-09-26T20:55:15Z
dc.date.available2023-09-26T20:55:15Z
dc.identifierTORREZAN-NITAO, Elis; FIGUEIREDO, Regina Celia Bressan Queiroz; MARQUES-SANTOS, Luis Fernando. Mitochondrial permeability transition pore in sea urchin female gametes. Mechanisms of Development, v. 154, p. 208-218, 2018.
dc.identifier0925-4773
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36979
dc.identifier10.1016/j.mod.2018.07.008
dc.identifier0925-4773
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8866181
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionMitochondrial permeability transition pore (MPTP) has been associated to calcium homeostasis and reactive oxygen species (ROS) generation in several cell types. While extensively investigated in somatic cells, there are few data regarding MPTP phenomenon in gametes. The aim of the present work was to investigate MPTP occurrence in sea urchin female gametes. The protonophores CCCP and FCCP, and the Ca2+ ionophore ionomycin, were used as pore inductors. Pore opening was monitored by mitochondrial potential sensitive probes and cobalt-quenched calcein assay. The pore desensitizer cyclosporin A (CsA) prevented the loss of mitochondrial inner membrane potential (ΔΨm) and pore opening induced by MPTP activators. The disruption of ΔΨm led to an increase in ROS generation, which was completely prevented by CsA. Our data also demonstrated that the increase in ROS production induced by MPTP opening requires extracellular Ca2+. In summary, the current study provides evidence about the occurrence of MPTP in sea urchin eggs in a similar manner as described in vertebrate somatic cells - CsA-sensitive, voltage- and Ca2+-triggered - and shows MPTP as a highly conserved physiological event through the evolution.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectCalcium
dc.subjectEggs
dc.subjectFemale gametes
dc.subjectMitochondria
dc.subjectMitochondrial permeability transition pore
dc.subjectSea urchin
dc.subjectAnimais
dc.subjectCálcio / metabolismo
dc.subjectCiclosporina / metabolismo
dc.subjectFeminino
dc.subjectCélulas Germinativas / metabolismo
dc.subjectPotencial da Membrana Mitocondrial / fisiologia
dc.subjectMitocôndrias / metabolismo
dc.subjectMitocôndrias / fisiologia
dc.subjectProteínas de Transporte da Membrana Mitocondrial / metabolismo
dc.subjectOuriços-do-Mar / metabolismo
dc.subjectOuriços-do-Mar / fisiologia
dc.titleMitochondrial permeability transition pore in sea urchin female gametes
dc.typeArticle


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