Article
Schistosoma mansoni Antigens Modulate Allergic Response In Vitro in Cells of Asthmatic Individuals
Registro en:
CARDOSO, L.C. et al. Schistosoma mansoni Antigens Modulate Allergic Response In Vitro in Cells of Asthmatic Individuals. Drug Development Research, v. 72, p. 538–548, 2011.
0272-4391
Autor
Cardoso, Luciana Santos
Oliveira, Sergio Costa
Souza, Ribson da Paixão de
Góes, Alfredo Miranda de
Oliveira, Ricardo Riccio
Alcântara, L. M
Almeida, M. C
Carvalho Filho, Edgar Marcelino
Araujo, Maria Ilma Andrade Santos
Resumen
Oliveira, Ricardo Riccio; Carvalho Filho, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. Brazilian National Research Council (CNPq). Schistosoma mansoni infection is associated with a low prevalence of asthma and a less
severe form of the disease. The mechanisms underlying this association may include the production of
regulatory cells and cytokines. The aim of this study was to evaluate the immune response induced by the
S. mansoni antigens, Sm22.6, PIII, and Sm29 and their ability to suppress allergen-specific IL-5 production
by peripheral blood mononuclear cells (PBMC) from asthmatic individuals. PBMCs were stimulated in
vitro with S. mansoni antigens in the presence or absence of antigen-1 of the mite Dermatophagoides
pteronyssinus (Der p1). Cytokines were measured in PBMC supernatants by enzyme-linked immunosorbent
assay (ELISA), and the phenotype of cells producing IL-10 was assessed using flow cytometry. High
production of S. mansoni antigen-specific IL-10 was observed not only in cells of S. mansoni-infected
individuals, but also in cells of noninfected asthmatic individuals. In the former group, the main cellular
sources of IL-10 were CD41 CD251, and CD141 cells. The levels of IFN-g, IL-5, and IL-13 in the
noninfected asthmatic group were 100 pg/ml in response to the antigens. Moreover, when S. mansoni
antigens were added to cultures stimulated with Der p1, levels of IL-10 were increased (Der
p152347118; Der p11Sm22.6511897595; Derp11PIII57997331; Derp11Sm2956527288 pg/
ml) with reduced levels of IL-5 (Der p152867219; Der p11Sm22.65937153; Derp11
PIII51327188; Derp11Sm29596786 pg/ml). The S. mansoni antigens evaluated in the present study
induced the production of the regulatory cytokine IL-10 and down-modulated the Th2 immune response
that participates in the pathology of asthma. Drug Dev Res 72:538–548, 2011.