Article
Specific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansi
Registro en:
QUEIROZ, Adriana O. et al. Specific Antibody Levels and Antigenic Recognition of Wistar Rats Inoculated with Distinct Isolates of Trypanosoma evansi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 7, p. 965-972, Oct. 2001.
0074-0276
10.1590/S0074-02762001000700014
1678-8060
Autor
Queiroz, Adriana O.
Legey, Ana P.
Xavier, Samanta C. C.
Jansen, Ana M.
Resumen
“Mal de Cadeiras”, an enzootic disease caused by Trypanosoma evansi, is one of the most important trypanosomiases in the Brazilian Pantanal region. The disease affects mainly horses, which are
widely used in extensive cattle production, an activity of greatest economical significance for the
region. The parasite also infects sylvan (coatis and capybaras) and domestic (dogs) animals, respectively considered wild and domestic reservoirs of T. evansi. For a better understanding of the interaction of T. evansi with its rodent host, we evaluated the differences in the specific antibody level patterns
and in the parasitic peptides recognition patterns of experimentally infected Wistar rats. The rats
experimentally infected with T. evansi isolates obtained from coatis, dogs and horses were submitted to
indirect immunofluorescence test (IgM e IgG) and Western blotting. The serological titers for IgM and
IgG ranged between 1:40 and 1:160. The most recognized polypeptide profiles were in a range of 17
and 74 kDa. Our data suggest that the humoral immune response in Wistar rats is not sufficient for
granting an effective control of T. evansi infections.