Article
Schistosoma antigens downmodulate the in vitro inflammatory response in individuals infected with human T cell lymphotropic virus type 1
Registro en:
LIMA, L. M. et al. Schistosoma antigens downmodulate the in vitro inflammatory response in individuals infected with human T cell lymphotropic virus type 1. Neuroimmunomodulation, v. 20, p. 233–238, 2013.
1021-7401
10.1159/000348700
Autor
Lima, Luciane Mota
Santos, Silvane Braga
Oliveira, Ricardo Riccio
Cardoso, Luciana Santos
Oliveira, Sérgio Costa
Góes, Alfredo Miranda
Loukas, Alex
Carvalho Filho, Edgar Marcelino
Araújo, Maria Ilma
Resumen
Oliveira, Ricardo Riccio; Carvalho Filho, Edgar Marcelino de “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. CNPQ (Universal 479417/2008 3) and NIH (R01AI079238A). Human T cell lymphotropic virus type 1 (HTLV-1) is the causal agent of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). While the immune response to HTLV-1 infection is polarized to the Th1-type, chronic helminth infections drive the Th2- and T regulatory-type, and are able to downregulate the inflammatory response in some autoimmune diseases. Objective: To evaluate whether
Schistosoma spp. antigens alter the in vitro cytokine
response in HTLV-1 infection. Methods: The recombinant
Schistosoma antigens Sm29 and ShTSP2 (tetraspanin) and
PIII, a fraction of the Schistosoma mansoni adult worm antigen
were added to peripheral blood mononuclear cell
(PBMC) cultures of HTLV-1-infected individuals and the levels
of interferon (IFN)-γ and interleukin (IL)-10 in the supernatants
were measured using the ELISA sandwich technique.
Results: Compared to the levels of cytokine in nonstimulated
cultures, the levels of IFN-γ were reduced in 50, 47 and 50% of patients by the presence of Sm29, ShTsp2 and PIII,
respectively. The downregulation of IFN-γ production in the
presence of Sm29 antigen was observed mainly in subjects
who had lower basal levels of this cytokine. The levels of
IL-10, however, increased by the addition of the three antigens
in the cultures in 74, 62 and 44% of individuals, respectively.
In addition, there was a decrease in the ratio of IFN-γ/
IL-10 levels in cultures stimulated with Sm29 and ShTSP2
when compared to nonstimulated ones. Conclusions: The
Schistosoma spp. antigens used in this study were able to
downmodulate IFN-γ production in vitro in HTLV-1 infection.
This may be associated with the increased levels of IL-10 induced
by the antigens.