The diagnosis of canine visceral leishmaniasis in Brazil: Confronting old problems

dc.creatorPessoa-e-Silva, Rômulo
dc.creatorVaitkevicius-Antão, Victor
dc.creatorAndrade, Thiago André Santos de
dc.creatorSilva, Anny Caroliny de Oliveira
dc.creatorOliveira, Gilsan Aparecida de
dc.creatorTrajano-Silva, Lays Adrianne Mendonça
dc.creatorNakasone, Eiji Kevin Nakasone
dc.creatorPaiva-Cavalcanti, Milena de
dc.date2019-10-03T13:42:05Z
dc.date2019-10-03T13:42:05Z
dc.date2019
dc.date.accessioned2023-09-26T20:53:02Z
dc.date.available2023-09-26T20:53:02Z
dc.identifierPESSOA-E-SILVA, Rômulo. et al. The diagnosis of canine visceral leishmaniasis in Brazil: Confronting old problems. Experimental Parasitology, v. 199, p. 9-16, Apr. 2019.
dc.identifier0014-4894
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36124
dc.identifier10.1016/j.exppara.2019.02.012
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8865562
dc.descriptionIn Brazil, the main strategy adopted to contain Visceral Leishmaniasis (VL) is the controversial culling of dogs with reagent serology for Canine VL (CVL). Despite there are studies showing that significant reduction of human cases has not been observed, as well as there are works demonstrating the occurrence of false-positive results in the confirmatory test, the protocol has been maintained. Researches that can reinforce the existence and persistence of this problem, as well as bring concrete alternatives are pivotal. In this context, the aim of this work was to evaluate and compare the serological, molecular and parasitological methods employed for CVL detection in Brazil, in dogs with diverse clinical profiles, from two endemic areas of Pernambuco state. Comparisons among TR-DPP®, EIE-LVC and qPCR (animals from Goiana-PE: 91) demonstrated that agreements varied from 'poor' to 'moderate' (kappa = 0.162-0.442), and a triple agreement occurred in 61.36% (54/88) of the samples. The highest percentage of agreement was obtained between TR-DPP® and EIE-LVC within the polysymptomatic group (93.33%; 14/15). Of the 34 dogs with reagent serology from Caruaru-PE, 17 (50%) and 29 dogs (85.29%) were positive for qPCR and parasitological exam, respectively. By comparing serology, qPCR and parasitological exam, the lowest percentage of agreements were obtained within the asymptomatic group (40%-72.72%). It was possible to observe that the percentage of agreement tended to decrease according to the absence of clinical manifestations in the dogs. Thus, from the fact that all diagnostic tools evaluated have their limitations, it is very important to be careful before to propose an alternative set of diagnostic criteria. Besides, the answer for better results in the control of CVL may not be in the choose of the best set of diagnostic tools, but it may be in the strategy of culling itself. In this context, it is very important to invest in alternative control measures, such as mass vaccination and treatment of dogs, thus reducing the transmission to the vector and helping to avoid new canine and human cases.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectCanine visceral leishmaniasis
dc.subjectLeishmaniasis
dc.subjectAnimais
dc.subjectAnticorpos Antiprotozoários / sangue
dc.subjectBrasil
dc.subjectDoença de Chagas / diagnóstico
dc.subjectDoença de Chagas / veterinária
dc.subjectReações Cruzadas
dc.subjectDNA de Protozoário / sangue
dc.subjectDoenças do Cão / diagnóstico
dc.subjectDoenças do Cão / parasitologia
dc.subjectDoenças do Cão / prevenção & controle
dc.subjectCães
dc.subjectEnsaio de Imunoadsorção Enzimática / veterinária
dc.subjectReações Falso-Negativas
dc.subjectReações Falso-Positivas
dc.subjectTécnica Indireta de Fluorescência para Anticorpo / veterinária
dc.subjectImunoensaio / veterinária
dc.subjectTécnicas Imunoenzimáticas / veterinária
dc.subjectLeishmania / imunologia
dc.subjectLeishmaniose Visceral / diagnóstico
dc.subjectLeishmaniose Visceral / parasitologia
dc.subjectLeishmaniose Visceral / prevenção & controle
dc.subjectLeishmaniose Visceral / veterinária
dc.subjectReação em Cadeia da Polimerase em Tempo Real / veterinária
dc.subjectTrypanosoma cruzi / imunologia
dc.titleThe diagnosis of canine visceral leishmaniasis in Brazil: Confronting old problems
dc.typeArticle


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