Histopathology and ultrastructure of midgut of Alabama argillacea (Hübner) (Lepidoptera: Noctuidae) fed Bt-cotton

dc.creatorSousa, Maria Esmeralda C.
dc.creatorSantos, Fábio A. B.
dc.creatorWanderley-Teixeira, Valéria
dc.creatorTeixeira, Alvaro A. C.
dc.creatorSiqueira, Herbert Álvaro A. de
dc.creatorAlves, Luiz C.
dc.creatorTorres, Jorge B.
dc.date2018-08-08T12:25:48Z
dc.date2018-08-08T12:25:48Z
dc.date2010
dc.date.accessioned2023-09-26T20:48:06Z
dc.date.available2023-09-26T20:48:06Z
dc.identifierSOUSA, Maria Esmeralda C. et al. Histopathology and Ultrastructure of Midgut of Alabama Argillacea (Hübner) (Lepidoptera: Noctuidae) Fed Bt-Cotton. Journal of Insect Physiology, v. 56, n. 12, p. 1913–1919, dez. 2010.
dc.identifier1879-1611
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/27999
dc.identifier10.1016/j.jinsphys.2010.08.014
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8864141
dc.descriptionTo CAPES for the concession of a scholarship to the first author, making this work possible.
dc.descriptionThe interaction of Cry toxins from Bacillus thuringiensis in the midgut of some insect larvae determines their efficacies as insecticides, due to the expression and availability of sites of action of the toxin in the midgut. Researches point out cases of resistance to Cry toxin due to alterations in the binding sites in columnar cell membrane. We analyzed the effects of Cry1Ac toxin expressed by Bt-cotton plants on Alabama argillacea midgut morphophysiology clarifying in levels of morphological and ultrastructural. Larvae in the 4th instar of A. argillacea after 20 min from ingesting Bt-cotton leaves expressing 0.183 ng of Cry1Ac exhibited ultrastructural and morphological modifications in the columnar cells with significant changes in the mitochondrial polymorphism, cytoplasmic vacuolization, microvillus and basal labyrinth. Expressive morphological alterations were also observed in the goblet cells indicating that the columnar cells are not the only target of the Cry1Ac toxin. The regenerative cells did not modify their structures and exhibited decrease in regeneration capacity. In conclusion, the ingestion of 0.183 ± 0.077 ng of Cry1Ac was enough to promote alterations in the columnar and goblet cells, besides reducing significantly the number of regenerative cells, which may have contributed to larval death. Nevertheless, further studies are necessary to determine the true cause of death.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectBollgard
dc.subjectLepidoptera
dc.subjectMicroscopia
dc.subjectLençol de algodoeiro
dc.subjectBacillus thuringiensis
dc.subjectBollgard
dc.subjectLepidoptera
dc.subjectMicroscopy
dc.subjectCotton leafworm
dc.subjectBacillus thuringiensis
dc.subjectAnimais
dc.subjectProteínas Bacterianas / administração & dosagem
dc.subjectProteínas Bacterianas / farmacologia
dc.subjectDistribuição Qui-Quadrado
dc.subjectSistema Digestivo / metabolismo
dc.subjectAparelho Digestivo / ultraestrutura
dc.subjectEndotoxinas / administração & dosagem
dc.subjectEndotoxinas / farmacologia
dc.subjectGossípio / metabolismo
dc.subjectProteínas Hemolisinas / administração & dosagem
dc.subjectProteínas de hemolisina / farmacologia
dc.subjectHistocitoquímica
dc.subjectLarva / metabolismo
dc.subjectLarva / ultraestrutura
dc.subjectLepidoptera / metabolismo
dc.subjectLepidópteros / ultraestrutura
dc.subjectMicroscopia Eletrônica
dc.subjectControle de Pragas, Biológico / métodos
dc.subjectPlantas Geneticamente Modificadas / metabolismo
dc.subjectPlantas Geneticamente Modificadas / Ultraestrutura
dc.titleHistopathology and ultrastructure of midgut of Alabama argillacea (Hübner) (Lepidoptera: Noctuidae) fed Bt-cotton
dc.typeArticle


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