Combination of flow cytometry and qPCR to study the immune response of american cutaneous leishmaniasis patients

dc.creatorBrelaz-de-Castro, Maria Carolina Accioly
dc.creatorAlmeida, Thays Miranda de
dc.creatorAlmeida, Amanda Ferreira de
dc.creatorSouza, Marina de Assis
dc.creatorMendes, Andresa Pereira de Oliveira
dc.creatorSilva, Rafael de Freitas E.
dc.creatorBrito, Maria Edileuza Felinto de
dc.creatorPereira, Valéria Rêgo Alves
dc.date2019-04-22T12:30:34Z
dc.date2019-04-22T12:30:34Z
dc.date2018
dc.date.accessioned2023-09-26T20:47:29Z
dc.date.available2023-09-26T20:47:29Z
dc.identifierBRELAZ-DE-CASTRO, M. C. A. et al. Combination of Flow Cytometry and QPCR to Study the Immune Response of American Cutaneous Leishmaniasis Patients. Microbial Pathogenesis, v. 123, p. 433–439, out. 2018.
dc.identifier1096-1208
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32648
dc.identifier10.1016/j.micpath.2018.07.044
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8863959
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionAmerican Cutaneous leishmaniasis (ACL) is a public health problem. The immunological response is mainly dependent on T cell cytokine responses and might influence disease presentation, susceptibility and development. The understanding of the host immune response role in the control and in the pathology of leishmaniasis is relevant and has implications on diagnosis, follow-up and vaccine development. In this study, the differences in the immune response and T cell profile of patients before treatment was investigated through flow cytometry and real time PCR in peripheral blood mononuclear cells after different antigenic stimulations. Among the main findings are the significant presence of TNF and IFN-γ gene expression after 24 h of in vitro stimulation, and 48 h later the presence of CD4+ T and CD8+ T cells producing IL-10 and IL-4. This may be due to the differences in cytokine release over time and the presence of cells other than lymphocytes influencing the mRNA transcript detection. Evaluation of the immune response of individuals with leishmaniasis or other diseases should associate different technologies and times points for a clear and more reliable assessment of the immune response. This would help in the design of vaccine strategies/immunotherapies.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectAmerican cutaneous leishmaniasis
dc.subjectCytokines
dc.subjectFlow cytometry
dc.subjectImmune response
dc.subjectLeishmania braziliensis
dc.subjectT lymphocytes
dc.subjectqPCR
dc.subjectAdolescente
dc.subjectAdulto
dc.subjectAntígenos, Protozoários / imunologia
dc.subjectBrasil
dc.subjectLinfócitos T CD4-Positivos / metabolismo
dc.subjectLinfócitos T CD8-Positivos / metabolismo
dc.subjectTécnicas de Cultura Celular
dc.subjectCitocinas / metabolismo
dc.subjectCitometria de Fluxo / métodos
dc.subjectHumanos
dc.subjectInterferon-gama / genética
dc.subjectInterferon-gamma / metabolismo
dc.subjectInterleucina-10 / metabolismo
dc.subjectInterleucina-4 / metabolismo
dc.subjectLeishmania braziliensis / imunologia
dc.subjectLeishmaniose cutânea / sangue
dc.subjectLeishmaniose cutânea / imunologia
dc.subjectLeucócitos Mononucleares / metabolismo
dc.subjectMeia idade
dc.subjectProteínas Protozoárias / imunologia
dc.subjectRNA mensageiro / metabolismo
dc.subjectReação em Cadeia da Polimerase em Tempo Real / métodos
dc.subjectLinfócitos T
dc.subjectAdulto jovem
dc.titleCombination of flow cytometry and qPCR to study the immune response of american cutaneous leishmaniasis patients
dc.typeArticle


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