Article
Peripheral effects induced in BALB/c mice infected with DENV by the intracerebral route
Registro en:
OLIVEIRA, E. R. A. et al. Peripheral effects induced in BALB/c mice infected with DENV by the intracerebral route. Virology, v.489, p.95-107, Feb. 2016.
0042-6822
10.1016/j.virol.2015.12.006
Autor
Oliveira, E. R. A.
Amorim, J. F. S
Paes, M. V.
Azevedo, A. S.
Gonçalves, A. J. S.
Costa, S. M.
Mantuano-Barradas, M.
Póvoa, T. F.
Meis, J.
Oliveira, C. A. Basílio de
Nogueira, A. C. M. A.
Alves, A. M. B.
Resumen
The lack of an immunocompetent animal model for dengue mimicking the disease in humans is a limitation for advances in this field. Inoculation by intracerebral route of neuroadapted dengue strains in mice is normally lethal and provides a straightforward readout parameter for vaccine testing. However, systemic effects of infection and the immune response elicited in this model remain poorly described. In the present work, BALB/c mice infected by the intracerebral route with neuroadapted DENV2 exhibited several evidences of systemic involvement. DENV-inoculated mice presented virus infective particles in the brain followed by viremia, especially in late stages of infection. Infection induced cellular and humoral responses, with presence of activated T cells in spleen and blood, lymphocyte infiltration and tissue damages in brain and liver, and an increase in serum levels of some pro-inflammatory cytokines. Data highlighted an interplay between the central nervous system commitment and peripheral effects under this experimental condition.