Article
Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection
Registro en:
KIKUTI, M. et al. Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection. Virology Journal, v. 15, p. 108, 2018.
1743-422X
10.1186/s12985-018-1015-6
Autor
Kikuti, Mariana
Tauro, Laura Beatriz
Moreira, Patrícia Sousa dos Santos
Campos, Gúbio Soares
Paploski, Igor Adolfo Dexheimer
Weaver, Scott C
Reis, Mitermayer Galvão dos
Kitron, Uriel
Ribeiro, Guilherme de Sousa
Resumen
Research Ethics Committee at the Gonçalo Moniz Institute, Oswaldo Cruz Foundation Serologic detection of Zika virus (ZIKV) infections is challenging because of antigenic similarities among flaviviruses. Objective: To evaluate the sensitivity and specificity of commercial ZIKV IgM and IgG enzyme-linked immunoassay
(ELISA) kits.
Methods: We used sera from febrile patients with RT-PCR-confirmed ZIKV infection to determine sensitivity and
sera from RT-PCR-confirmed dengue cases and blood donors, both of which were collected before ZIKV epidemics
in Brazil (2009–2011 and 2013, respectively) to determine specificity.
Results: The ZIKV IgM-ELISA positivity among RT-PCR ZIKV confirmed cases was 0.0% (0/14) and 12.5% (1/8) for
acute- and convalescent-phase sera, respectively, while its specificity was 100.0% (58/58) and 98.3% (58/59) for
acute- and convalescent-phase sera of dengue patients, and 100.0% (23/23) for blood donors. The ZIKV IgG-ELISA
sensitivity was 100.0% (6/6) on convalescent-phase sera from RT-PCR confirmed ZIKV patients, while its specificity
was 27.3% (15/55) on convalescent-phase sera from dengue patients and 45.0% (9/20) on blood donors’ sera. The
ZIKV IgG-ELISA specificity among dengue confirmed cases was much greater among patients with primary dengue
(92.3%; 12/13), compared to secondary dengue (7.1%; 3/42).
Conclusions: In a setting of endemic dengue transmission, the ZIKV IgM-ELISA had high specificity, but poor
sensitivity. In contrast, the ZIKV IgG-ELISA showed low specificity, particularly for patients previously exposed to
dengue infections. This suggests that this ZIKV IgM-ELISA is not useful in confirming a diagnosis of ZIKV infection in
suspected patients, whereas the IgG-ELISA is more suitable for ZIKV diagnosis among travelers, who reside in areas
free of flavivirus transmission, rather than for serosurveys in dengue-endemic areas.