Arthropod-borne infections are dependent on environmental conditions, and several combinations of natural and human-related variables play an important role in vector populations aswell as the life cycle of agents carried by the arthropods. The top 5 canine arthropod-transmitted agents, Dirofilaria immitis, Leishmania spp., Ehrlichia canis, Anaplasma phagocytophilum, and Borrelia burgdorferi infect unprotected animals without propensity. The purpose of this study was to determine the prevalence of these parasite species in three different landscape settings (sandbanks, plains and mountains) along a 60-km line. During a 6-month period, blood samples were collected from dogs (N12 months old) living in the different settings. Prevalence of D. immitis was determined by modified Knott test and ELISA. Prevalence of E. canis, A. phagocytophilum, and B. burgdorferi was determined by ELISA, and Leishmania spp. by ELISA, indirect immunofluorescence, and immunocromatographic assays. D. immitis was most prevalent in the sandbank (68.9%) as well as Leishmania spp. (34.5%), and tick-transmitted agents, A. phagocytophilum and E. canis in the plains (61.7%). B. burgdorferi was not detected. Depending on the resources for arthropods present in regions, dogs are likely to be exposed to different arthropod-borne parasites and should receive preventives tailored to the risk of infection in the region in which the dog resides.