Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil

dc.creatorMachado, Lais Ceschini
dc.creatorMorais-Sobral, Mariana Carolina de
dc.creatorCampos, Tulio de Lima
dc.creatorPereira, Mylena Ribeiro
dc.creatorAlbuquerque, Maria de Fátima Pessoa Militão de
dc.creatorGilbert, Clément
dc.creatorFranca, Rafael Freitas Oliveira
dc.creatorWallau, Gabriel Luz
dc.date2019-12-16T13:05:01Z
dc.date2019-12-16T13:05:01Z
dc.date2019
dc.date.accessioned2023-09-26T20:39:53Z
dc.date.available2023-09-26T20:39:53Z
dc.identifierMACHADO, Lais Ceschini et al. Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. PLoS Neglected Tropical Diseases, v. 13, n. 5, p. 1-15, 2019.
dc.identifier1935-2727
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/37962
dc.identifier10.1371/journal.pntd.0007332
dc.identifier1935-2727
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8861584
dc.descriptionChikungunya virus (CHIKV) is an RNA virus from the Togaviridae family transmitted by mosquitoes in both sylvatic and urban cycles. In humans, CHIKV infection leads to a febrile illness, denominated Chikungunya fever (CHIKF), commonly associated with more intense and debilitating outcomes. CHIKV arrived in Brazil in 2014 through two independent introductions: the Asian/Caribbean genotype entered through the North region and the African ECSA genotype was imported through the Northeast region. Following their initial introduction, both genotypes established their urban cycle among large naive human populations causing several outbreaks in the Americas. Here, we sequenced CHIKV genomes from a recent outbreak in the Northeast region of Brazil, employing an in-house developed Next-Generation Sequencing (NGS) protocol capable of directly detecting multiple known CHIKV genotypes from clinical positive samples. Our results demonstrate that both Asian/Caribbean and ECSA genotypes expanded their ranges, reaching cocirculation in the Northeast region of Brazil. In addition, our NGS data supports the findings of simultaneous infection by these two genotypes, suggesting that coinfection might be more common than previously thought in highly endemic areas. Future efforts to understand CHIKV epidemiology should thus take into consideration the possibility of coinfection by different genotypes in the human population.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectGenome sequencing
dc.subjectMultiple chikungunya virus genotypes
dc.subjectChikungunya
dc.subjectAdulto
dc.subjectIdoso
dc.subjectBrasil / epidemiologia
dc.subjectFebre de Chikungunya / epidemiologia
dc.subjectFebre de Chikungunya / virologia
dc.subjectVírus Chikungunya / classificação
dc.subjectVírus Chikungunya / genética
dc.subjectVírus Chikungunya / isolamento & purificação
dc.subjectCoinfecção / epidemiologia
dc.subjectCoinfecção / virologia
dc.subjectSurtos de Doenças
dc.subjectFeminino
dc.subjectGenoma Viral
dc.subjectGenótipo
dc.subjectHumanos
dc.subjectMasculino
dc.subjectPessoa de Meia-Idade
dc.subjectFilogenia
dc.subjectPolimorfismo de Nucleotídeo Único
dc.subjectSequenciamento Completo do Genoma
dc.subjectAdulto Jovem
dc.titleGenome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil
dc.typeArticle


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