Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation

dc.creatorPereira, Paula Fabiana Santos
dc.creatorSilva, Ana Carolina Alves de Paula e
dc.creatorPimentel, Bruna Natália Alves da Silva
dc.creatorPinatti, Ivo Mateus
dc.creatorSimões, Alexandre Zirpoli
dc.creatorVergani, Carlos Eduardo
dc.creatorVieira, Débora Ferreira Barreto
dc.creatorSilva, Marcos Alexandre Nunes da
dc.creatorMiranda, Milene Dias
dc.creatorMonteiro, Maria Eduarda Santos
dc.creatorTucci, Amanda
dc.creatorDoñate‑Buendía, Carlos
dc.creatorMínguez‑Vega, Gladys
dc.creatorAndrés, Juan
dc.creatorLongo, Elson
dc.date2022-06-14T12:43:30Z
dc.date2022-06-14T12:43:30Z
dc.date2022
dc.date.accessioned2023-09-26T20:39:36Z
dc.date.available2023-09-26T20:39:36Z
dc.identifierPEREIRA, Paula Fabiana Santos et al. Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation. Scientific Reports, v. 12, 8118, p. 1-18, 2022.
dc.identifier2045-2322
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/53245
dc.identifier10.1038/s41598-022-11902-5
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8861498
dc.descriptionIn the current COVID-19 pandemic, the next generation of innovative materials with enhanced anti-SARS-CoV-2 activity is urgently needed to prevent the spread of this virus within the community. Herein, we report the synthesis of chitosan/α-Ag2WO4 composites synthetized by femtosecond laser irradiation. The antimicrobial activity against Escherichia coli, Methicilin-susceptible Staphylococcus aureus (MSSA), and Candida albicans was determined by estimating the minimum inhibitory concentration (MIC) and minimal bactericidal/fungicidal concentration (MBC/MFC). To assess the biocompatibility of chitosan/α-Ag2WO4 composites in a range involving MIC and MBC/MFC on keratinocytes cells (NOK-si), an alamarBlue™ assay and an MTT assay were carried out. The SARSCoV- 2 virucidal effects was analyzed in Vero E6 cells through viral titer quantified in cell culture supernatant by PFU/mL assay. Our results showed a very similar antimicrobial activity of chitosan/α- Ag2WO4 3.3 and 6.6, with the last one demonstrating a slightly better action against MSSA. The chitosan/α-Ag2WO4 9.9 showed a wide range of antimicrobial activity (0.49–31.25 μg/mL). The cytotoxicity outcomes by alamarBlue™ revealed that the concentrations of interest (MIC and MBC/ MFC) were considered non-cytotoxic to all composites after 72 h of exposure. The Chitosan/α-Ag2WO4 (CS6.6/α-Ag2WO4) composite reduced the SARS-CoV-2 viral titer quantification up to 80% of the controls. Then, our results suggest that these composites are highly efficient materials to kill bacteria (Escherichia coli, Methicillin-susceptible Staphylococcus aureus, and the yeast strain Candida albicans), in addition to inactivating SARS-CoV-2 by contact, through ROS production.
dc.formatapplication/pdf
dc.languageeng
dc.publisherNature
dc.rightsopen access
dc.subjectSARS‑CoV‑2
dc.subjectCOVID-19
dc.subjectInativação do SARS-CoV-2
dc.subjectComposto quitosana/α-Ag2WO4
dc.subjectGerado por laser
dc.subjectPandemia
dc.subjectSARS‑CoV‑2
dc.subjectCOVID-19
dc.subjectInactivation of SARS‑CoV‑2
dc.subjectChitosan/α‑Ag2WO4 composite
dc.subjectSecond laser irradiation
dc.subjectPandemic
dc.titleInactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation
dc.typeArticle


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