Article
Comparison between Precipitin and ELISA Tests in the Bloodmeal Detection of Aedes aegypti (Linnaeus) and Aedes fluviatilis (Lutz) Mosquitoes Experimentally Fed on Feline, Canine and Human Hosts
Registro en:
GOMES, Luciana A. M. et al. Comparison between Precipitin and ELISA Tests in the Bloodmeal Detection of Aedes aegypti (Linnaeus) and Aedes fluviatilis (Lutz) Mosquitoes Experimentally Fed on Feline, Canine and Human Hosts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 5, p. 693-695, July 2001.
0074-0206
10.1590/S0074-02762001000500020
1678-8060
Autor
Gomes, Luciana A. M.
Duarte, Rosemere
Lima, Dinair C.
Diniz, Beatriz Sanson
Serrão, Maria Lucia
Labarthe, Norma
Resumen
The identification of arthropod bloodmeals is important in many epidemiological studies, as, the
understanding of the life cycle of vectors and the patogens they transmit, as well as helping to define
arthropods’ control strategies. The precipitin test has been used for decades, but ELISA is slowly becoming more popular. To compare the two tests for sensitivity, specificity and accuracy to detect small
insect bloodmeals, Aedes aegypti or Ae. fluviatilis mosquitoes were fed either on feline, canine or human hosts. Mosquitoes were frozen at 6, 12, 24, 48 or 72 h after feeding. Precipitin test showed better
specificity and accuracy and ELISA test showed higher sensitivity. Better results with both tests were
achieved when mosquitoes were frozen within 48 h from feeding.