eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei

dc.creatorFreire, Eden R.
dc.creatorVashisht, Ajay A.
dc.creatorMalvezzi, Amaranta M.
dc.creatorZuberek, Joanna
dc.creatorLangousis, Gerasimos
dc.creatorSaada, Edwin A.
dc.creatorNascimento, Janaína de F.
dc.creatorStepinski, Janusz
dc.creatorDarzynkiewicz, Edward
dc.creatorHill, Kent
dc.creatorMelo Neto, Osvaldo P. de
dc.creatorWohlschlegel, James A.
dc.creatorSturm, Nancy R.
dc.creatorCampbell, David A.
dc.date2017-11-22T14:14:50Z
dc.date2017-11-22T14:14:50Z
dc.date2014
dc.date.accessioned2023-09-26T20:36:01Z
dc.date.available2023-09-26T20:36:01Z
dc.identifierFREIRE, E. R. et al. eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei. RNA (New York, N.Y.), v. 20, n. 8, p. 1272–1286, ago. 2014.
dc.identifier1469-9001
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/23309
dc.identifier10.1261/rna.045534.114
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8860345
dc.descriptionMembers of the eIF4E mRNA cap-binding family are involved in translation and the modulation of transcript availability in other systems as part of a three-component complex including eIF4G and eIF4A. The kinetoplastids possess four described eIF4E and five eIF4G homologs. We have identified two new eIF4E family proteins in Trypanosoma brucei, and define distinct complexes associated with the fifth member, TbEIF4E5. The cytosolic TbEIF4E5 protein binds cap 0 in vitro. TbEIF4E5 was found in association with two of the five TbEIF4Gs. TbIF4EG1 bound TbEIF4E5, a 47.5-kDa protein with two RNA-binding domains, and either the regulatory protein 14-3-3 II or a 117.5-kDa protein with guanylyltransferase and methyltransferase domains in a potentially dynamic interaction. The TbEIF4G2/TbEIF4E5 complex was associated with a 17.9-kDa hypothetical protein and both 14-3-3 variants I and II. Knockdown of TbEIF4E5 resulted in the loss of productive cell movement, as evidenced by the inability of the cells to remain in suspension in liquid culture and the loss of social motility on semisolid plating medium, as well as a minor reduction of translation. Cells appeared lethargic, as opposed to compromised in flagellar function per se. The minimal use of transcriptional control in kinetoplastids requires these organisms to implement downstream mechanisms to regulate gene expression, and the TbEIF4E5/TbEIF4G1/117.5-kDa complex in particular may be a key player in that process. We suggest that a pathway involved in cell motility is affected, directly or indirectly, by one of the TbEIF4E5 complexes.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectProteínas 14-3-3
dc.subjectCinetoplasto
dc.subjectPAC mRNA
dc.subjectmotilidade social
dc.subjectFator de iniciação da tradução
dc.subject14-3-3 protein
dc.subjectkinetoplastid
dc.subjectmRNA cap
dc.subjectsocial motility
dc.subjecttranslation initiation factor
dc.subjectFator de Iniciação 4E em Eucariotos
dc.subjectmetabolismo
dc.subjectFator de Iniciação 4G em Eucariotos
dc.subjectmetabolismo
dc.subjectProteínas de Ligação ao Cap de RNA
dc.subjectmetabolismo
dc.subjectProcessamento Pós-Transcricional do RNA
dc.subjectSequência de aminoácidos
dc.subjectCapuzes de RNA
dc.subjectmetabolismo
dc.subjectTécnicas de Inativação de Genes
dc.subjectHumanos
dc.subjectDados de Sequência Molecular
dc.subjectLigação Proteica
dc.subjectRNA de Protozoário
dc.subjectmetabolismo
dc.titleeIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei
dc.typeArticle


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