Article
Proteomic Profile of Brucella abortus-Infected Bovine Chorioallantoic Membrane Explants
Registro en:
MOL, Juliana P. S. et al. Proteomic Profile of Brucella abortus- Infected Bovine Chorioallantoic Membrane Explants. Plos One, v.11, n.4, e0154209, 16p, Apr. 2016.
1932-6203
10.1371/journal.pone.0154209
Autor
Mol, Juliana P. S.
Pires, Simone F.
Chapeaurouge, Alexander D.
Perales, Jonas
Santos, Renato L.
Andrade, Hélida M.
Lage, Andrey P.
Resumen
Brucella abortus is the etiological agent of bovine brucellosis, a zoonotic disease that causes significant economic losses worldwide. The differential proteomic profile of bovine chorioallantoic membrane (CAM) explants at early stages of infection with B. abortus (0.5, 2, 4, and 8 h) was determined. Analysis of CAM explants at 0.5 and 4 h showed the highest differences between uninfected and infected CAM explants, and therefore were used for the Differential Gel Electrophoresis (DIGE). A total of 103 spots were present in only one experimental group and were selected for identification by mass spectrometry (MALDI/ToF-ToF). Proteins only identified in extracts of CAM explants infected with B. abortus were related to recognition of PAMPs by TLR, production of reactive oxygen species, intracellular trafficking, and inflammation.