Article
Influence of the HPA axis on the inflammatory response in cutaneous wounds with the use of 670-nm laser photobiomodulation.
Registro en:
VILELA, D. D. C. et al. Influence of the HPA axis on the inflammatory response in cutaneous wounds with the use of 670-nm laser photobiomodulation. Journal of Photochemistry and Photobiology: Biology, v. 116, p. 114-120, 2012.
1873-2682
dx.doi.org/10.1016/j.jphotobiol.2012.05.018
Autor
Vilela, Deyla Duarte Carneiro
Chamusca, Flávia Villela
Andrade, Jorge Clarêncio de Souza
Vallve, Maria de Lourdes Farre
Gonzalez, Ana Cristina
Andrade, Zilton de Araújo
Medrado, Alena Ribeiro Alves Peixoto
Reis, Sílvia Regina de Almeida
Resumen
This study evaluated the influence of hypothalamic–pituitary–adrenal (HPA) axis in cutaneous wounds
subjected to laser biomodulation. A total of 48 rats were divided into two groups: Group I (GI) with 24
adrenalectomized animals and Group II (GII) with 24 non-adrenalectomized animals. Each group was
divided into two subgroups: the irradiated subgroup which laser was applied to four points at the edges
of the wound (670 nm laser, 9 mW) and control subgroup. Rats in each subgroup were sacrificed at 24 or
72 h. Adrenal glands were only removed from GI rats. Three days after adrenalectomy, a cutaneous
wound was made. An immunohistochemical analysis was performed using anti-CD45 and anti-CD8 antibodies.
Flow cytometry was used to count T lymphocytes and their subpopulations in blood. Decreases in
the number of CD45-positive inflammatory cells and in the total numbers of CD8- and CD45-positive cells
were observed in histological sections of adrenalectomized animals subjected to laser biomodulation at
24 h. Similar results were observed for distribution of total lymphocytes in blood (p < 0.05). The action of
670 nm laser does not depend exclusively on HPA axis. It is believed that corticosteroid-promoting
enzymes liberated in non-adrenal tissues may influence immune response under the influence of this
type of phototherapy.