Domestic swine in a visceral leishmaniasis endemic area produce antibodies against multiple Leishmania infantum antigens but apparently resist to L. infantum infection.

dc.creatorSilva, Evandro Moraes
dc.creatorAntunes, Fabiana Rodrigues
dc.creatorRodrigues, Márcio Silva
dc.creatorJulião, Fred da Silva
dc.creatorLima, Artur Gomes Dias
dc.creatorSousa, Valderez Lemos de
dc.creatorAlcantara, Adriano Costa de
dc.creatorReis, Eliana Almeida Gomes
dc.creatorNakatani, Maria
dc.creatorBadaró, Roberto José da Silva
dc.creatorReis, Mitermayer Galvão dos
dc.creatorPontes-de-Carvalho, Lain Carlos
dc.creatorFranke, Carlos Roberto
dc.date2014-04-28T16:58:56Z
dc.date2014-04-28T16:58:56Z
dc.date2006
dc.date.accessioned2023-09-26T20:31:15Z
dc.date.available2023-09-26T20:31:15Z
dc.identifierSILVA, E. M. et al. Domestic swine in a visceral leishmaniasis endemic area produce antibodies against multiple Leishmania infantum antigens but apparently resist to L. infantum infection. Acta Tropica, v. 98, n. 2, p. 176-182, 2006.
dc.identifier0001-706X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7550
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8858705
dc.descriptionIn order to investigate whether pigs can be infected by Leishmania infantum, a serological and parasitological study was carried out on swine in the Jequi´e municipality, Northeast of Brazil. Anti-Leishmania infantum antibodies were detected in 37 out of 92 swine (40.2%), by two different assays: an anti-L. infantum lysate and an anti-K39 recombinant protein ELISA. An experimental study was also carried out to verify the susceptibility of domestic pigs to L. infantum infection. Three sows inoculated with 108 stationary-phase infective L. infantum promastigotes (26% metacyclic promastigotes) per kilogram of body weight produced anti- Leishmania antibodies until the end of the experiment, 11 months later. No parasites, however, could be visualized through optical microscopy of spleen, liver and bone marrowor by in vitro culture of these organs. Homogenates of these organs were also inoculated in hamsters, without producing infection. No Leishmania DNA was detected by polymerase chain reaction (PCR) in sand flies fed on these animals. The results indicate that domestic pigs bitten by L. infantum-infected vectors in the endemic area do not display a full infection pattern, and the positive association in endemic areas between the presence of swine and infection in canines may not be ascribable to the former acting as a parasite reservoir
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectVisceral leishmaniasis
dc.subjectPig
dc.subjectImmune response; Experimental infection
dc.subjectAnticorpos Antiprotozoários/biossíntese
dc.subjectLeishmania infantum/isolamento & purificação
dc.subjectLeishmaniose Visceral/diagnóstico
dc.subjectDoenças dos Suínos/parasitologia
dc.subjectAnimais
dc.subjectAnticorpos Antiprotozoários/sangue
dc.subjectAntígenos de Protozoários/química
dc.subjectWestern Blotting/veterinária
dc.subjectBrasil/epidemiologia
dc.subjectCricetinae
dc.subjectDNA de Protozoário/genética
dc.subjectEnsaio de Imunoadsorção Enzimática/veterinária
dc.subjectFeminino
dc.subjectLeishmania infantum/genética
dc.subjectLeishmania infantum/imunologia
dc.subjectLeishmaniose Visceral/epidemiologia
dc.subjectReação em Cadeia da Polimerase/veterinária
dc.subjectProteínas de Protozoários/química
dc.subjectEstudos Soroepidemiológicos
dc.subjectDoenças dos Suínos/epidemiologia
dc.titleDomestic swine in a visceral leishmaniasis endemic area produce antibodies against multiple Leishmania infantum antigens but apparently resist to L. infantum infection.
dc.typeArticle


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