In Brazil, since 2009, there has been an ever increasing widespread of the blaKPC-2 gene, mainly in Klebsiella pneumoniae. This study aims to assess the molecular epidemiology and genetic background of this gene in Enterobacteriaceae (non–K. pneumoniae) species from9 Brazilian states between 2009 and 2011. Three hundred eighty-seven isolateswere analyzed exhibiting nonsusceptibility to carbapenems, inwhich the blaKPC-2 gene was detected in 21.4%. By disk diffusion and E-test, these isolates exhibited high rates of resistance to most of the antimicrobials tested, including tigecycline (45.6% nonsusceptible) and polymyxin B (16.5%), the most resistant species being Enterobacter aerogenes and Enterobacter cloacae. We found great clonal diversity and a variety of blaKPC-2–carrying plasmids, all of them exhibiting a partial Tn4401 structure. Therefore, this study demonstrates the dissemination of KPC-2 in 9 Enterobacteriaceae species, including species thatwere not previously described such as Pantoea agglomerans and Providencia stuartii.