Article
Evidence for associations between the purinergic receptor P2X7 (P2RX7) and toxoplasmosis
Registro en:
JAMIESON, Sarra E. et al. Evidence for associations between the purinergic receptor P2X7 (P2RX7) and toxoplasmosis. Genes Immun., v. 11, n. 5, p. 374-383, July 2010.
1466-4879
Autor
Jamieson, Sarra E.
Rangel, Alba Lucinia Peixoto
Hargrave, Aubrey C.
Roubaix, Lee-Anne de
Mui, Ernest J.
Boulter, Nicola R.
Miller, E. Nancy
Fuller, Stephen J.
Wiley, James S.
Castellucci, Léa
Boyer, Kenneth
Peixe, Ricardo Guerra
Kirisits, Michael J.
Elias, Liliani de Souza
Coyne, Jessica J.
Oliveira, Rodrigo Correa de
Sautter, Mari
Smith, Nicholas C.
Lees, Michael P.
Swisher, Charles N.
Heydemann, Peter
Noble, A. Gwendolyn
Patel, Dushyant
Bardo, Dianna
Burrowes, Delilah
McLone, David
Roizen, Nancy
Withers, Shawn
Oliveira, Lilian Maria Garcia Bahia de
McLeod, Rima
Blackwell, Jenefer M.
Resumen
Congenital Toxoplasma gondii infection can result in intracranial calcification, hydrocephalus, and retinochoroiditis. Acquired infection is commonly associated with ocular disease. Pathology is characterized by strong pro-inflammatory responses. Ligation of ATP by purinergic receptor P2X7, encoded by P2RX7, stimulates pro-inflammatory cytokines and can lead directly to killing of intracellular pathogens. To determine whether P2X7 plays a role in susceptibility to congenital toxoplasmosis, we examined polymorphisms at P2RX7 in 149 child/parent trios from North America. We found association (FBAT Z scores ±2.429; P= 0.015) between the derived C(+)G(−) allele (f= 0.68; OR= 2.06; 95% CI: 1.14–3.75) at SNP rs1718119 (1068T>C; Thr-348-Ala), and a second synonymous variant rs1621388 in linkage disequilibrium with it, and clinical signs of disease per se. Analysis of clinical sub-groups showed no association with hydrocephalus, with effect sizes for associations with retinal disease and brain calcifications enhanced (OR=3.0 to 4.25; 0.004<P<0.009) when hydrocephalus was removed from the analysis. Association with toxoplasmic retinochoroiditis was replicated (FBAT Z scores ±3.089; P= 0.002) in a small family-based study (60 families; 68 affected offspring) of acquired infection in Brazil, where the ancestral T(+) allele (f= 0.296) at SNP rs1718119 was strongly protective (OR= 0.27; 95% CI: 0.09–0.80)