DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis

dc.creatorSilva, Robson Amaro Augusto da
dc.creatorTavares, Natalia Machado
dc.creatorCosta, Dirceu Joaquim
dc.creatorPitombo, Maiana A
dc.creatorBarbosa, Larissa
dc.creatorFukutani, Kiyoshi Ferreira
dc.creatorMiranda, José Carlos
dc.creatorOliveira, Camila Indiani de
dc.creatorValenzuela, Jesus G
dc.creatorBarral, Aldina Maria Prado
dc.creatorSoto, Manuel
dc.creatorBarral Netto, Manoel
dc.creatorBrodskyn, Claudia Ida
dc.date2014-02-10T14:08:38Z
dc.date2014-02-10T14:08:38Z
dc.date2011
dc.date.accessioned2023-09-26T20:29:06Z
dc.date.available2023-09-26T20:29:06Z
dc.identifierSILVA, R. R. A. da et al. DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis. Acta Tropica, v. 120, n. 3, p. 185-190, 2011.
dc.identifier0001-706X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7306
dc.identifier10.1016/j.actatropica.2011.08.007
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8857929
dc.descriptionIt was recently shown that immunization of hamsters with DNA plasmids coding LJM19, a sand fly salivary protein, partially protected against a challenge with Leishmania chagasi, whereas immunization with KMP11 DNA plasmid, a Leishmania antigen, induced protection against L. donovani infection. In the present study, we evaluated the protective effect of immunization with both LJM19 and KMP11 DNA plasmid together. Concerning the protection against an infection by L. chagasi, immunization with DNA plasmids coding LJM19 or KMP11, as well as with both plasmids combined, induced IFN-γ production in draining lymph nodes at 7, 14 and 21 days post-immunization. Immunized hamsters challenged with L. chagasi plus Salivary Gland Sonicate (SGS) from Lutzomyia longipalpis showed an enhancement of IFN-γ/IL-10 and IFN-γ/TGF-ß in draining lymph nodes after 7 and 14 days of infection. Two and five months after challenge, immunized animals showed reduced parasite load in the liver and spleen, as well as increased IFN-γ/IL-10 and IFN-γ/TGF-ß ratios in the spleen. Furthermore, immunized animals remained with a normal hematological profile even five months after the challenge, whereas L. chagasi in unimmunized hamsters lead to a significant anemia. The protection observed with LJM19 or KMP11 DNA plasmids used alone was very similar to the protection obtained by the combination of both plasmids
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectHamster
dc.subjectLeishmania chagasi
dc.subjectVisceral leishmaniasis
dc.subjectSaliva
dc.subjectDNA plasmids
dc.subjectProtection
dc.subjectProteínas de Insetos/imunologia
dc.subjectLeishmaniose Visceral/prevenção & controle
dc.subjectGlicoproteínas de Membrana/imunologia
dc.subjectProteínas de Protozoários/imunologia
dc.subjectPsychodidae/imunologia
dc.subjectProteínas e Peptídeos Salivares/imunologia
dc.subjectVacinas de DNA/imunologia
dc.subjectAnimais
dc.subjectCricetinae
dc.subjectFeminino
dc.subjectProteínas de Insetos/genética
dc.subjectInterferon gama/secreção
dc.subjectInterleucina-10/secreção
dc.subjectLeishmaniose Visceral/imunologia
dc.subjectFígado/parasitologia
dc.subjectLinfonodos/imunologia
dc.subjectMasculino
dc.subjectGlicoproteínas de Membrana/genética
dc.subjectMesocricetus
dc.subjectProteínas Recombinantes/administração & dosagem
dc.subjectBaço/imunologia
dc.subjectBaço/parasitologia
dc.subjectFator de Crescimento Transformador beta/secreção
dc.titleDNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis
dc.typeArticle


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