L-asparaginase isolated from Streptomyces ansochromogenes promotes Th1 profile and activates CD8+ T cells in human PBMC: an in vitro investigation

dc.creatorLacerda, G. R. da Silva
dc.creatorMelo, C. M. L. de
dc.creatorSoares, A. K. de Araújo
dc.creatorMoreira, L. R.
dc.creatorCoriolano, M. C.
dc.creatorLima, G. M. de Souza
dc.creatorNapoleão, T. H.
dc.creatorLorena, V. M. B.de
dc.creatorSilva, L. A. de Oliveira da
dc.creatorNascimento, S. C. do
dc.date2019-05-22T16:08:10Z
dc.date2019-05-22T16:08:10Z
dc.date2018
dc.date.accessioned2023-09-26T20:27:54Z
dc.date.available2023-09-26T20:27:54Z
dc.identifierSILVA, G. R. da Silva et al. L-Asparaginase Isolated from Streptomyces Ansochromogenes Promotes Th1 Profile and Activates CD8+ T Cells in Human PBMC: An in Vitro Investigation. Journal of Applied Microbiology, v. 124, n. 5, p. 1122–1130, May 2018.
dc.identifier1365-2672
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33180
dc.identifier10.1111/jam.13644
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8857503
dc.descriptionAims:A new L-asparaginase produced byStreptomyces ansochromogenesUFPEDA 3420 actinobacteria was used in this study against humanlymphocyte cultures to evaluate the immunological profile induced by thisenzyme.Methods and Results:Cultures of lymphocytes were stimulated withS.ansochromogenesL-asparaginase, and cytotoxicity, cell viability, cell stimulationand cytokine production were analysed. This newS. ansochromogenesL-asparaginase induced activation and proliferation of the TCD8+lymphocytesubset and produced higher TNF-a, IFN-c, IL-2 and IL-10 levels in a 24-hassay.Conclusion:Streptomyces ansochromogenesL-asparaginase is a promisingmolecule to be used inin vivomodels and to deepen preclinical tests againstacute lymphoblast leukaemia.Significance and Impact of Study:L-asparaginase is an indispensablecomponent of the chemotherapeutic treatment of acute lymphoblast leukaemia(ALL) and acute myeloid leukaemia (AML). Currently, drugs such asAsparaginaseâ, Kidrolaseâ, and Elsparâand Erwinaseâare efficient againstleukemic disease, but promote immunosuppression and other side effects inhuman organisms. Our purifiedS. ansochromogenesL-asparaginase showedpromissory results inducing,in vitro, higher immunostimulation in humanPBMC, especially in T CD8+lymphocyte subsets.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectAcute lymphoblast leukaemia
dc.subjectImmunomodulation
dc.subjectL-asparaginase
dc.subjectLymphocytes
dc.subjectStreptomyces ansochromogenes
dc.subjectAsparaginase / isolamento e purificação
dc.subjectAsparaginase / farmacologia
dc.subjectAsparaginase / toxicidade
dc.subjectLinfócitos T CD8-Positivos / efeitos de drogas
dc.subjectLinfócitos T CD8-Positivos / imunologia
dc.subjectHumanos
dc.subjectLeucócitos Mononucleares / efeitos de drogas
dc.subjectLeucócitos Mononucleares / imunologia
dc.subjectAtivação de Linfócitos / efeitos de drogas
dc.subjectStreptomyces / enzimologia
dc.subjectTh1 Células / efeitos de drogas
dc.titleL-asparaginase isolated from Streptomyces ansochromogenes promotes Th1 profile and activates CD8+ T cells in human PBMC: an in vitro investigation
dc.typeArticle


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