Article
Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae)
Registro en:
PITA, Sebastián et al. Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 116, e210259, p. 1 - 12, 2021.
0074-0206
10.1590/0074-02760210259
Autor
Pita, Sebastián
Gómez-Palacio, Andrés
Lorite, Pedro
Dujardim, Jean Pierre
Chavez, Tamara
Villacís, Anita G.
Galvão, Cleber
Calleros, Lucía
Pereyra-Mello, Santiago
Burgueño-Rodríguez, Gabriela
Panzera, Francisco
Resumen
BACKGROUND Panstrongylus rufotuberculatus (Hemiptera-Reduviidae) is a triatomine species with a wide geographic
distribution and a broad phenotypic variability. In some countries, this species is found infesting and colonising domiciliary
ecotopes representing an epidemiological risk factor as a vector of Trypanosoma cruzi, etiological agent of Chagas disease. In
spite of this, little is known about P. rufotuberculatus genetic diversity.
METHODS Cytogenetic studies and DNA sequence analyses of one nuclear (ITS-2) and two mitochondrial DNA sequences (cyt
b and coI) were carried out in P. rufotuberculatus individuals collected in Bolivia, Colombia, Ecuador and Mexico. Moreover, a
geometric morphometrics study was applied to Bolivian, Colombian, Ecuadorian and French Guiana samples.
OBJECTIVES To explore the genetic and phenetic diversity of P. rufotuberculatus from different countries, combining chromosomal
studies, DNA sequence analyses and geometric morphometric comparisons.
FINDINGS We found two chromosomal groups differentiated by the number of X chromosomes and the chromosomal position of
the ribosomal DNA clusters. In concordance, two main morphometric profiles were detected, clearly separating the Bolivian sample
from the other ones. Phylogenetic DNA analyses showed that both chromosomal groups were closely related to each other and
clearly separated from the remaining Panstrongylus species. High nucleotide divergence of cyt b and coI fragments were observed
among P. rufotuberculatus samples from Bolivia, Colombia, Ecuador and Mexico (Kimura 2-parameter distances higher than 9%).
MAIN CONCLUSIONS Chromosomal and molecular analyses supported that the two chromosomal groups could represent
different closely related species. We propose that Bolivian individuals constitute a new Panstrongylus species, being necessary
a detailed morphological study for its formal description. The clear morphometric discrimination based on the wing venation
pattern suggests such morphological description might be conclusive.