Article
Activation of anaerobic metabolism in Biomphalaria glabrata (Mollusca: Gastropoda) experimentally infected by Angiostrongylus cantonensis (Nematoda, Metastrongylidae) by high-performance liquid chromatography
Registro en:
ALVES, Vinicius Menezes Tunholi et al. Activation of anaerobic metabolism in Biomphalaria glabrata (Mollusca: Gastropoda) experimentally infected by Angiostrongylus cantonensis (Nematoda, Metastrongylidae) by high-performance liquid chromatography. Parasitology International, n.63, p. 64–68, 2014.
10.1016/j.parint.2013.09.004
Autor
Alves, Vinícius Menezes Tunholi
Tunholi, Victor Menezes
Castro, Rosane N
Sant`Ana, Luiza D`Oliveira
Amaral, Luciana Santos
Oliveira, Ana Paula Martins de
Garcia, Juberlan
Thiengo, Silvana Carvalho
Pinheiro, Jairo
Maldonado Jr., Arnaldo
Resumen
The activity of lactate dehydrogenase and the concentrations of glucose in the hemolymph and of glycogen in the
digestive gland and cephalopedal mass of Biomphalaria glabrata experimentally infected with Angiostrongylus
cantonensis were evaluated. Additionally, high performance liquid chromatography (HPLC) was used to determine
the hemolymph concentrations of some carboxylic acids (oxalic, piruvic, lactic and succinic). After one,
two and three weeks of infection, the snails were dissected to collect the hemolymph and separate the tissues.
A significant reduction of the levels of glucose in the hemolymph was observed as of the first week of infection
in relation to the control group. The lactate dehydrogenase activity of the infected group was significantly higher
than the average of the control group. This increase was accompanied by a reduction of the levels of piruvic acid
and an increase in the levels of lactic acid in the hemolymph of the parasited snails, confirming the acceleration of
the anaerobic metabolism, necessary for the host to obtain energy and maintain its redox balance. In parallel,
there was a decrease in the glycogen content of the storage tissues, with that reduction being significantly greater
in the cephalopedal mass than the digestive gland, demonstrating that in this interaction system, the mobilization
of glycogen was not sufficient to maintain and reestablish the normal glycemia of the infected snails.