Article
Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection
Registro en:
MESQUITA, Rafael D. et al. Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection. PNAS - Proceedings of the National Academy of Sciences, v. 112, n. 48, p. 14936-14941, Dec. 2015.
1091-6490
10.1073/pnas.1600205113
Autor
Mesquita, Rafael D.
Amaral, Raquel J. Vionette
Lowenberger, Carl
Rivera-Pomar, Rolando
Monteiro, Fernando A.
Minx, Patrick
Spieth, John
Carvalho, A. Bernardo
Panzera, Francisco
Lawson, Daniel
Torres, André Q.
Gondim, Katia C.
Granzotto, Adriana
Guarneri, Alessandra A.
Guigó, Roderic
Harry, Myriam
Hughes, Daniel S. T. H
Jablonka, Willy
Jacquin-Joly, Emmanuelle
Juaréz, M. Patricia
Koerich, Leonardo B.
Lorenzo, Marcelo G.
Lange, Angela B.
Latorre-Estivalis, José Manuel
Lavore, Andrés
Lawrence, Gena G.
Lazoski, Cristiano
Lazzari, Claudio R.
Lopes, Raphael R.
Lugon, Magda D.
Majerowicz, David
Marcet, Paula L.
Mariotti, Marco
Masuda, Hatisaburo
Megy, Karine
Melo, Ana C. A.
Missirlis, Fanis
Mota, Theo
Pagola, Lucia
Noriega, Fernando G.
Nouzova, Marcela
Nunes, Rodrigo D.
Oliveira, Raquel L. L.
Silveira, Gilberto Oliveira
Ons, Sheila
Orchard, Ian
Silva, Gabriela O. Paiva
Pascual, Agustina
Pavan, Marcio G.
Pedrini, Nicolás
Peixoto, Alexandre A.
Pereira, Marcos H.
Pike, Andrew
Polycarpo, Carla
Prosdocimi, Francisco
Cardoso, Livia Silva
Rodrigues, Rodrigo Ribeiro
Robertson, Hugh M.
Salerno, Ana Paula
Salmon, Didier
Santesmasses, Didac
Schama, Renata
Seabra Junior, Eloy S.
Silva Neto, Mario A. C.
Gomes, Matheus Souza
Sterkel, Marcos
Taracena, Mabel L.
Tojo, Marta
Tu, Zhijian Jake
Tubio, Jose M. C.
Ursic-Bedoya, Raul
Venancio, Thiago M.
Ribeiro, Jose M. C.
Nuno, Ana Beatriz Walter
Wilson, Derek
Warren, Wesley C.
Wilson, Richard K.
Huebner, Erwin
Dotson, Ellen M.
Oliveira, Pedro L.
Sorgine, Marcos H. F.
Waterhouse, Robert M.
Montague, Michael J.
Abad-Franch, Fernando
Bezerra, Michele Alves
Amaral, Laurence R.
Araujo, Helena M.
Araujo, Ricardo N.
Aravind, L.
Atella, Georgia C.
Azambuja, Patricia
Berni, Mateus
Cunha, Paula R. Bittencourt
Braz, Gloria R. C.
Calderón-Fernández, Gustavo
Carareto, Claudia M. A.
Christensen, Mikkel B.
Costa, Igor R.
Costa, Samara G.
Dansa, Marilvia
Daumas Filho, Carlos R. O.
Paula, Iron F. de
Dias, Felipe A.
Dimopoulos, George
Emrich, Scott J.
Esponda-Behrens, Natalia
Fampa, Patricia
Fernandez-Medina, Rita D.
Fonseca, Rodrigo N. da
Fontenele, Marcio
Fronick, Catrina
Fulton, Lucinda A.
Gandara, Ana Caroline
Garcia, Eloi S.
Genta, Fernando A.
Giraldo-Calderón, Gloria I.
Gomes, Bruno
Resumen
O artigo apresenta nas duas primeiras páginas nota de correção. Rhodnius prolixus not only has served as a model organism for the
study of insect physiology, but also is a major vector of Chagas disease,
an illness that affects approximately seven million people worldwide.
We sequenced the genome of R. prolixus, generated assembled
sequences covering 95% of the genome (∼702 Mb), including 15,456
putative protein-coding genes, and completed comprehensive genomic
analyses of this obligate blood-feeding insect. Although immunedeficiency
(IMD)-mediated immune responses were observed, R. prolixus
putatively lacks key components of the IMD pathway, suggesting
a reorganization of the canonical immune signaling network. Although
both Toll and IMD effectors controlled intestinal microbiota,
neither affected Trypanosoma cruzi, the causal agent of Chagas disease,
implying the existence of evasion or tolerance mechanisms.
R. prolixus has experienced an extensive loss of selenoprotein genes,
with its repertoire reduced to only two proteins, one of which is a
selenocysteine-based glutathione peroxidase, the first found in insects.
The genome contained actively transcribed, horizontally transferred
genes from Wolbachia sp., which showed evidence of codon use evolution
toward the insect use pattern. Comparative protein analyses
revealed many lineage-specific expansions and putative gene absences
in R. prolixus, including tandem expansions of genes related to chemoreception,
feeding, and digestion that possibly contributed to the
evolution of a blood-feeding lifestyle. The genome assembly and these
associated analyses provide critical information on the physiology and
evolution of this important vector species and should be instrumental
for the development of innovative disease control methods.