Article
In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract
Registro en:
DALTRO, Sérgio Ricardo Teixeira et al. In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract. Planta Médica, 2020.
0032-0943
10.1055/a-1237-4268
Autor
Daltro, Sérgio Ricardo Teixeira
Santos, Ivanilson Pimenta
Barros, Paula Ladeia
Moreira, Diogo Rodrigo Magalhães
Tomassini, Therezinha Coelho Barbosa
Ribeiro, Ivone Maria
Santos, Ricardo Ribeiro dos
Meira, Cássio Santana
Soares, Milena Botelho Pereira
Resumen
CNPq (grant number 562655/
2010-7), PRONEX (grant number 0002/2014) and FAPESB (grant number
0042/2013) The need for new immunomodulatory drugs is due to the side
effects associated with the prolonged use of the currently
used immunomodulatory drugs. In this context, the present
work aimed to investigate the immunomodulatory effect of
an ethanolic concentrated extract from Physalis angulata. The
cytotoxicity of samples was determined using peritoneal macrophages
though the Alamar Blue assay. The immunomodulatory
activity of the ethanolic extract from P. angulata on activated
macrophages was determined by measurement of
nitrite and cytokine production. The immunosuppressive effects
of the ethanolic extract from P. angulata was evaluated
on lymphocyte proliferation and cytokine production. The effects
of the extract on cell cycle progression and cell death on
lymphocytes were evaluated by flow cytometry. Lastly, the
ethanolic extract from P. angulata was tested in vivo in toxicological
tests and in models of peritonitis and delayed-type hypersensitivity
response. The ethanolic extract from P. angulata
decreased nitrite, interleukin-6, interleukin-12, and TNF-α
production by activated macrophages without affecting the
cell viability. In addition, the ethanolic extract from P. angulata
inhibited lymphoproliferation and the secretion of interleukin-
2, interleukin-6, and IFN-γ, and increased interleukin-4 secretion
by activated splenocytes. Flow cytometry analysis in lymphocyte
cultures showed that treatment with the ethanolic
extract from P. angulata induces cell cycle arrest in the G1
phase followed by cell death by apoptosis. Moreover, mice
treated with the extract from P. angulata at 100 or 200mg/kg
did not show signs of toxicity or alterations in serum components.
Finally, the ethanolic extract from P. angulata significantly
reduced neutrophil migration and reduced paw edema
in bovine serum albumin-induced the delayed-type hypersensitivity
responsemodel. Our results demonstrate the potential
of the ethanolic extract of P. angulata as an alternative for the
treatment of immune-inflammatory diseases.