Article
Comparison of the performance of enzyme immunoassays for hepatitis B and C detection in dried blood spot
Registro en:
CRUZ, Helena Medina et al. Comparison of the performance of enzyme immunoassays for hepatitis B and C detection in dried blood spot. Journal of Immunoassay and Immunochemistry, v. 39, n. 2, p. 228-233, 2018.
1532-1819
10.1080/15321819.2018.1470095
: 1532-4230
Autor
Cruz, Helena Medina
Cruz, Juliana Custódio Miguel
Silva, Elisangela Ferreira da
Portilho, Moyra Machado
Marques, Vanessa Alves
Lewis-Ximenez, Lia Laura
Lampe, Elisabeth
Villar, Livia Melo
Resumen
Dried blood spots (DBSs) could be an alternative to serum for hepatitis B virus (HBV) and hepatitis C virus (HCV) diagnosis. This study aims to evaluate two enzyme immunoassays (EIAs) for HBsAg and anti-HCV detection using DBS. Serum was tested using commercial EIA. DBS was tested using optimized EIA developed for serum and commercial EIA developed for DBS (Imunoscreen). Concordances between DBS and serum samples for both markers and EIAs were higher than 97%. Both EIAs demonstrated good performance for HBsAg and anti-HCV detection using DBS, and these methods could be used unchangeably increasing the access for HBV and HCV diagnosis. 2022-01-01