Article
Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA
Registro en:
SOUZA, Carolina Senra Alves de et al. Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA. BMC Res Notes, v. 11, n. 1, 455, 2018. doi: 10.1186/s13104-018-3571-7.
1756-0500
10.1186/s13104-018-3571-7
Autor
Souza, Carolina Senra Alves de
Gomes, Luciana Inácia
Siqueira, Liliane Maria Vidal
Coelho, Paulo Marcos Zech
Rabello, Ana Lúcia Teles
Oliveira, Edward José de
Resumen
Objective: We developed a laboratorial platform to release a commercial platform used in the PCR-ELISA for the molecular diagnosis of schistosomiasis mansoni. On following, PCR-ELISA platform laboratorial was evaluated in 206 feces samples collected of individual living in a Brazilian low endemicity area.
Results: The PCR-ELISA laboratorial platform indicated a prevalence rate of 25.2%, which was higher than the Kato-Katz technique (18.4%) and lower than the commercial platform (30.1%). Considering Kato-Katz technique as the reference, there were 97.4% and 91.1% of relative sensitivity and specificity rates, respectively. The laboratorial platform presented good precision, performance diagnostic, and can be used in replacement to the commercial platform for diagnosis of schistosomiasis by PCR-ELISA.