Article
In vitro cultured peripheral blood mononuclear cells from patients with chronic schistosomiasis mansoni show immunomodulation of cyclin D1,2,3 in the presence of soluble egg antigens
Registro en:
AZEVEDO, Ana Carolina Campi et al. In vitro cultured peripheral blood mononuclear cells from patients with chronic schistosomiasis mansoni show immunomodulation of cyclin D1,2,3 in the presence of soluble egg antigens. Microbes and Infection, v. 9, n. 12-13, p. 1493-1499, 2007.
1769-714X
10.1016/j.micinf.2007.08.004
Autor
Azevedo, Ana Carolina Campi
Gazzinelli, Giovanni
Bottazzi, Maria Elena
Carvalho, Andréa Teixeira de
Oliveira, Rodrigo Corrêa de
Caldas, Iramaya Rodrigues
Resumen
Infection with Schistosoma mansoni induces a wide range of effects on the immune responses of the host. In the present study we investigated the influence of soluble egg antigens (SEA) on the cell cycle of peripheral blood mononuclear cells (PBMC) from infected and non-infected individuals with S. mansoni resident in an endemic area and blood donors from non-endemic area. The cell cycle, the expression of activation markers and cyclin D+1,2,3 CD3+ frequency was assessed by flow cytometry. Stimulation of PBMC from infected patients with SEA resulted in a lower frequency of CD3+ T cells in S phase when compared with the non-infected group. In addition, infected patients presented a decrease of activation marker expression (CD69+, HLA-DR+ and CD28− on CD4+ cells and CD25+, HLA-DR+ on CD8+ cells). A reduced frequency was observed of cyclin D1,2,3 expression in SEA-stimulated T cells from infected individuals when compared with those from the non-infected group. The decreased expression of activation markers and frequency of cyclin D1,2,3 in T cells may result in arrest of T cells in the G0/G1 phase of the cell cycle, thus explaining the down-regulation observed in chronic schistosomiasis. 2025-01-01